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  • R3251 - D-Ribulose-5-phosphate 3-Epimerase from baker's yeast (S. cerevisiae)

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R3251 Sigma

D-Ribulose-5-phosphate 3-Epimerase from baker's yeast (S. cerevisiae)

lyophilized powder, 50-100 units/mg protein (modified Warburg-Christian)

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Properties

Related Categories 5.1.x.x Racemases and epimerases, 5.x.x.x Isomerases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
form   lyophilized powder
foreign activity   phosphoriboisomerase, alcohol dehydrogenase, transketolase, and transaldolase <0.1%
storage temp.   −20°C

Description

Unit Definition

One unit will convert 1 μmole of D-ribulose 5-phosphate to D-xylulose 5-phosphate per min at pH 7.7 at 25°C when coupled with transketolase, α-glycerophosphate dehydrogenase, and triosephosphate isomerase.

Physical form

Lyophilized and essentially sulfate-free; contains approx. 35% citrate buffer salts

Application

D-Ribulose-5-phosphate 3-Epimerase is an enzyme that converts the reversible conversion of D-ribulose 5-phosphate into D-xylulose 5-phosphate, which is important for the cellular response against oxidative stress . D-Ribulose-5-phosphate 3-Epimerase is involved in the pentose phosphate pathway, pentose and glucuronate interconversions and carbon fixation. Product R3251 is from baker’s yeast and is provided as a lyophilized powder. It is useful in enzyme systems requiring low sulfate.

Biochem/physiol Actions

RPE is a metalloenzyme and has been shown to use the divalent Zn2+ ion predominantly for catalysis. Human D-ribulose-5-phosphate 3-epimerase (hRPE) has been shown to use Fe2+ for catalysis .

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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