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R3400 Sigma

Monoclonal Anti-c-K-Ras antibody produced in mouse

clone 234-4.2, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   mouse, human, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10 μg/mL using normal skin tissue
  immunoprecipitation: suitable
  western blot: suitable
clone   234-4.2, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2κ
mol wt   antigen mol wt 21 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... KRAS(3845)
mouse ... Kras(16653)
rat ... Kras(24525)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant p21 protein

Physical form

Solution in 0.05 M sodium phosphate buffer, pH7.5, containing 0.2% gelatin and < 0.1% sodium azide

Specificity

The antibody reacts with c-K-Ras and v-K-Ras p21. It crossreacts with cH-Ras and cN-Ras p21 on immunoblots.

Preparation Note

Purified using protein A or G

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Price and Availability


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Safety & Documentation

Safety Information

Personal Protective Equipment  dust mask type N95 (US), Eyeshields, Gloves
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Oncogenes and Tumor Suppressors Reprogram Metabolism

Proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication. This requires a reprogramming of the metabolic pathways to ensure nutrients s...
Keywords: Aerobic, Antitumor agents, Apoptosis, Biofiles, Cancer, Citric Acid Cycle, Degradations, Environmental, Events, Gene expression, Glycolysis, Growth factors, Metabolic Pathways, Metabolism, Metabolites, Nucleotide Synthesis, PAGE, Pentose phosphate pathway, Phosphorylations, Support

esiRNA Knockdown Efficiency Tested by Western Blotting

A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
Keywords: Cell proliferation, Gene expression, PAGE, Reductions, Transfection, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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