• USA Home
  • R4018 - Ribonucleic acid, transfer, phenylalanine specific from brewer's yeast

EMAIL THIS PAGE TO A FRIEND
R4018 Sigma

Ribonucleic acid, transfer, phenylalanine specific from brewer's yeast

Synonym: Yeast Phe tRNA, tRNA

Purchase

Properties

grade   for molecular biology
  for molecular biology
form   lyophilized powder
solubility   water: soluble2  mg/mL
storage temp.   2-8°C

Description

Components

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

General description

Transfer RNA is isolated from brewer’s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine.

The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.

The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.

This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.

Preparation Note

Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).

Application

Suitable for use:
• As a tRNA standard
• As a substrate in tRNA synthatase assays
• As a substrate in other reactions involving tRNAs

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C
R5636 Ribonucleic acid, transfer from baker's yeast (S. cerevisiae), buffered aqueous solution
R9001 Ribonucleic acid, transfer from baker's yeast (S. cerevisiae), Type X, lyophilized powder
R8508 Ribonucleic acid, transfer from baker's yeast (S. cerevisiae), buffered aqueous solution
R7876 Ribonucleic acid, transfer from wheat germ, Type V, 15-19 unit/mg solid

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?