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| Related Categories | Molecular Biology, Nucleic Acid Amplification, PCR/Amplification, Plant Biotechnology, Plant Molecular Biology, |
| form | solution |
| usage | sufficient for ≥600 reactions |
| concentration | 100 × |
| storage temp. | 2-8°C |
Sigma′s Reference Dye for qPCR is a proprietary dye for use with real-time PCR. It is used for normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.
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