|Related Categories||Molecular Biology, PCR/Amplification, Quantitative PCR, Reagents, Traditional QPCR More...|
|usage||sufficient for ≥600 reactions|
Sigma′s Reference Dye for qPCR is a proprietary dye for use with real-time PCR. It is used for normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.
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The single most important step in assuring success with PCR is high quality DNA preparation. Integrity and purity of DNA template is essential. Quantitative PCR involves multiple rounds of enzymatic ...
Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Error, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Help, Instructions, Legal, Melting, Methods, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Sequences, Size-exclusion chromatography, Solvents, Support, The Reporter, Titrations, Type
Sambrook, J. et al. Molecular Cloning: A Laboratory Manual, (2000) 3rd
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