• USA Home
  • R4642 - Ribonuclease A from bovine pancreas

EMAIL THIS PAGE TO A FRIEND
R4642 Sigma

Ribonuclease A from bovine pancreas

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

Purchase

Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
mol wt   mol wt 13.7 kDa
  mol wt ~13,700
concentration   20-40 mg/mL
suitability   suitable for
foreign activity   Endonuclease and exonuclease, none detected
  NICKase and DNase, none detected
storage temp.   −20°C

Description

Components

RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).

Analysis Note

Protein determined by E1%/280.

Application

Suitable for:
• RNase protection assays
• Removal of unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolysis of RNA contained in protein samples
• Plasmid DNA purification

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Features and Benefits

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA

General description

RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

Other Notes

Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.

Unit Definition

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.

Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
Product R4642, Ribonuclease A from bovine pancreas (RNase A), is a solution.  What does the solution contain?
The product is supplied in a solution containing 50% glycerol and 10 mM Tris-HCl, pH 8.0.  The solution formulation information can be viewed on the Product Datasheet by clicking on the following link:   http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/r4642dat.Par.0001.File.tmp/r4642dat.pdf
What is the shelf life of Product R4642, Ribonuclease A from bovine pancreas (RNase A)? 
This product is stable for at least 2 years when stored properly at –20 °C.  The shelf life information can be found on the Product Datasheet by accessing the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/r4642dat.Par.0001.File.tmp/r4642dat.pdf
When using Product R4642, Ribonuclease A from bovine pancreas (RNase A), do I need to boil the RNase A to inactivate residual DNases?
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity.  This information can be viewed on the Product Information Sheet by accessing the following link:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/r4642dat.Par.0001.File.tmp/r4642dat.pdf
What is the concentration of Product R4642, Ribonuclease A from bovine pancreas (RNase A)?
The concentration is lot specific and can be found on the Certificate of Analysis on our website by accessing the following link:  http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/R4642
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 – Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
Show more questions
Protocols & Articles

Articles

HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections

From our library of Articles, Sigma-Aldrich presents HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptides and Proteins on Proteomix® SCX-NP1.7, 3 cm x 4.6 mm I.D. Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides and Proteins on Proteomix® SCX-NP1.7, 3 cm x 4.6 mm I.D. Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® SCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® SCX-NP5
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins Demonstrating Selectivity Differences between TSKgel® Strong (SP-5PW) and Weak (CM-5PW) Cation Exchangers

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Demonstrating Selectivity Differences between TSKgel® Strong (SP-5PW) and Weak (CM-5PW) Cation Exchangers
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins and Peptides on Proteomix® WCX-NP1.7 Demonstrating Lot-to-Lot Reproducibility

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins and Peptides on Proteomix® WCX-NP1.7 Demonstrating Lot-to-Lot Reproducibility
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® and SRT® SEC-300, Comparison of Particle Size and Column I.D.

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® and SRT® SEC-300, Comparison of Particle Size and Column I.D.
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on Antibodix® WCX-NP10, Comparison of WCX Columns

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Antibodix® WCX-NP10, Comparison of WCX Columns
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX-NP1.7, Demonstration of Lot-to-Lot Reproducibility

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX-NP1.7, Demonstration of Lot-to-Lot Reproducibility
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX-NP5
Keywords: High performance liquid chromatography, Separation

HPLC Analysis of Proteins on Proteomix® WCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® WCX-NP5
Keywords: High performance liquid chromatography, Proteomics

HPLC Analysis of Proteins on TSKgel® BioAssist S, Ion Selectivity Comparison

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® BioAssist S, Ion Selectivity Comparison
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins on TSKgel® BioAssist S

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® BioAssist S
Keywords: High performance liquid chromatography, Proteomics

HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of a Protein Mixture on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of a Protein Mixture on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
Keywords: Chromatography, Clinical, High performance liquid chromatography, Size-exclusion chromatography

Loading Capacity of RNaseA on Proteomix® SCX-NP3

From our library of Articles, Sigma-Aldrich presents Loading Capacity of RNaseA on Proteomix® SCX-NP3
Keywords: Chromatography, High performance liquid chromatography

Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size

From our library of Articles, Sigma-Aldrich presents Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins

From our library of Articles, Sigma-Aldrich presents Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins
Keywords: Chromatography, High performance liquid chromatography

Protocols

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity. The Spectrophotometric Stop Rate Determination [Absorbance at 300 nm (A300), Light path = 1 cm] is based on the foll...

GenElute Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. The purified DNA is greater than 20 kb in length and can be used in...
Keywords: AGE, Amplification, Cell disruption, Centrifugation, Electrophoresis, Evaporation, Filtration, Gel electrophoresis, Molecular biology, Phase transitions, Polymerase chain reaction, Precipitation, Purification

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

D5758 Diethyl pyrocarbonate, ≥97% (NMR)

related product

Product #

Description

Add to Cart

P6911 Protease from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)
LIG1 DNA Ligation Kit
D9380 DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution
R0884 T7 RNA Polymerase, recombinant, expressed in E. coli, buffered aqueous solution
D8276 DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution
D2886 DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution
P4978 Phosphatase, Alkaline from calf intestine, buffered aqueous glycerol solution
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C
T4427 Terminal Transferase from calf thymus, buffered aqueous glycerol solution
P4390 Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution
E1131 Exonuclease III from Escherichia coli BE25 /psGR3, buffered aqueous glycerol solution
G5663 Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
D7291 Deoxyribonuclease I RNase-free solution from bovine pancreas
T7428 Topoisomerase I from wheat germ, buffered aqueous glycerol solution
R6513 Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?