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R4642 Sigma

Ribonuclease A from bovine pancreas

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
mol wt   mol wt 13.7 kDa
  mol wt ~13,700
concentration   20-40 mg/mL
suitability   suitable for
foreign activity   Endonuclease and exonuclease, none detected
  NICKase and DNase, none detected
storage temp.   −20°C



RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).

Analysis Note

Protein determined by E1%/280.


Suitable for:
• RNase protection assays
• Removal of unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolysis of RNA contained in protein samples
• Plasmid DNA purification

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Features and Benefits

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA

General description

RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

Other Notes

Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.

Unit Definition

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.

Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

Price and Availability

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Enzymatic Assay of Ribonuclease A (EC

This procedure may be used for determination of Ribonuclease A (RNase A) activity. The Spectrophotometric Stop Rate Determination [Absorbance at 300 nm (A300), Light path = 1 cm] is based on the foll...

GenElute Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

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