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R5773 Sigma

Anti-Raf-1 (631-648) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
species reactivity   rat, human
application(s)   immunoprecipitation: 5 μg using 10 μg lysate of human epidermal carcinoma A431 cells
  microarray: suitable
  western blot: 1:8,000 using a human epidermal carcinoma A431 cell line lysate
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 74 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... RAF1(5894)
rat ... Raf1(24703)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 631-648 of human Raf-1.

General description

Raf-1 or c-Raf belongs to the Raf family of kinases that have regulatory functions in cell growth, differentiation, growth factor receptor stimulation via the MAPK signalling. The other members of the family are A-Raf and B-Raf. Raf-1 is widely expressed in many tissues while A-Raf and B-Raf are mainly expressed in urogenital and brain tissues. All the isoforms have unique as well as overlapping regulatory functions. c-Raf kinase is the first identified downstream effector kinase of Ras, and is the activator of MEK which in turn activates ERK. Phosphorylation of c-Raf at Ser338 and Tyr340 regulates its biological function.
Anti-Raf-1 specifically recognises Raf-1 (74 kDa). The antibody may also detect Raf-1 degradation products at 40-60 kDa.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Specificity

The sequence of the immunizing peptide is identical in rat Raf-1 and v-Raf from the mouse sarcoma virus.

Application

By immunoblotting, a minimum working dilution of 1:8,000 is recommended using a lysate of the human epidermal carcinoma A431 cell line. The antibody may be used in immunoprecipitation of Raf-1 using 5 μg IgG with Protein A-agarose and 10 μg lysate of cultured A431 cells. The antibody may be used for detection of Raf-1 in rat brain cells , mouse spermatozoa and NIH3T3 mouse fibroblast cells.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
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Peer-Reviewed Papers
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References

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