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R5884 Sigma

Nsi I from Neisseria sicca

Restriction Enzyme

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   buffered aqueous glycerol solution
concentration   10,000 units/mL
shipped in   wet ice
storage temp.   −20°C

Description

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Linkage

Isoschizomer: Ava III

Specificity

Recognition sequence: 5′-ATGCA/T-3′
Heat inactivation: Inactivated at 65 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50 % glycerol (v/v), 0.02% polydocanol (v/v), 0.01% gelatine (v/v), at 4 °C

Application

NsiI is a restriction endonuclease used in molecular biology to cleave DNA at the recognition site 5′-ATGCA/T-3′, generating DNA fragments with 3′-cohesive ends.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Restriction Enzyme Buffer Reference

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer ...
Keywords: Buffers, Digestions, Enzyme activity, PAGE, Solid phase extractions

Protocols

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Restriction Enzyme Digest Protocol

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also...
Keywords: Buffers, Digestions, Purification

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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B3657 Restriction Enzyme Buffer SH, 10 ×

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