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R6260 Sigma

Monoclonal Anti-CD8−Quantum Red antibody produced in mouse

clone UCHT-4, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD8

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   UCHT-4, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
shipped in   wet ice
storage condition   protect from light
storage temp.   2-8°C
Gene Information   human ... CD8A(925), CD8B(926)
biological source   mouse
conjugate   Quantum Red® conjugate

Description

Immunogen

human thymocytes followed by peripheral blood T cells.

Antigen Background

CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567

Preparation Note

Quantum Red is a tandem fluorescent dye created by coupling R-phycoerythrin with Cy5 reactive dye. This red dye is efficiently excited at 488 nm and emits at 670 nm.

Legal Information

Quantum Red is a trademark of Sigma-Aldrich Co. LLC

Texas Red is a registered trademark of Life Technologies

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
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