|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Core Bioreagents,|
|grade||for molecular biology|
|form||buffered aqueous glycerol solution|
|shipped in||wet ice|
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Comment: Avoid suboptimal reaction conditions such as low salt, high pH (>8.0) and high glycerol (>5%) as they will alter EcoRI specificity and precipitate star activity.
Recognition sequence: 5′-G/AATTC-3′
Cutting results: a 2-10-fold EcoR I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 20 minutes.
Solution in 10 mM Tris-HCl, pH 7.2 , 1 mM EDTA, 200 mM NaCl , 0.5 mM dithioerythritol, 50% glycerol (v/v), 0.2% Triton X- 100 (v/v), 4°C
EcoRI is a restriction endonuclease that is used in molecular biology applications to cleave DNA at the recognition site 5′-G/AATTC-3′, generating fragments with 5′-cohesive termini.
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From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
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CYP1AI and CYP2E1 gene polymorphisms may increase susceptibility to Oral Submucous Fibrosis among betel quid chewers of Eastern India. Chaudhuri SR, Mukherjee S, Paul RR, et al. Gene 513(2), 268-71, (2013)
A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases. Podgórska B, Kujawska G, Skurzewski M, et al. Acta Biochim. Pol. 59(4), 669-72, (2012)
Relationship of BsmI polymorphism of vitamin D receptor gene with left ventricular hypertrophy and atherosclerosis in hemodialysis patients. El-Shehaby AM, El-Khatib MM, Marzouk S, et al. Scand. J. Clin. Lab. Invest. 73(1), 75-81, (2013)
Interactions between serum vitamin D levels and vitamin D receptor gene FokI polymorphisms for renal function in patients with type 2 diabetes. Yokoyama K, Nakashima A, Urashima M, et al. PLoS ONE 7(12), e51171, (2012)
The association between BsmI polymorphism and bone mineral density in young patients with epilepsy who are taking phenytoin. Phabphal K, Geater A, Limapichart K, et al. Epilepsia 54(2), 249-55, (2013)
Double-strand break repair by homologous recombination in primary mouse somatic cells requires BRCA1 but not the ATM kinase. Kass EM, Helgadottir HR, Chen CC, et al. Proc. Natl. Acad. Sci. U. S. A. 110(14), 5564-9, (2013)
Dopamine D2 receptor and ankyrin repeat and kinase domain containing 1 genes among the two linguistically distinct tribal populations of Ranchi district, Jharkhand, India. Sachdeva MP, Saksena D, Mondal PR, et al. Anthropol. Anz. 70(1), 15-26, (2013)
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