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R6379 Sigma

Xho I from Xanthomonas holcicola

Restriction Enzyme

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Description

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Specificity

Recognition sequence: 5′-C/TCGAG-3′
Cutting results: a 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Polydocanol (v/v) at 4 °C

Application

XhoI is a resteriction endonuclease used for molecular biology methods to cut DNA molecules at the recognition sequence 5′-C/TCGAG-3′ to generate fragments with 5′-cohesive ends.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Restriction Enzyme Digest Protocol

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also...
Keywords: Buffers, Digestions, Purification

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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B3657 Restriction Enzyme Buffer SH, 10 ×

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