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  • R6400 - Anti-phospho-Retinoblastoma (pSer807,811) antibody produced in rabbit

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R6400 Sigma

Anti-phospho-Retinoblastoma (pSer807,811) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, rat, human
application(s)   western blot (chemiluminescent): 1:1,000 using human fibroblasts synchronized by serum deprivation
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... RB1(5925)
mouse ... Rb1(19645)
rat ... Rb1(24708)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to residues around phosphoserine 807/811 of the human retinoblastoma tumor suppressor protein (Rb), conjugated to KLH.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

Specificity

Recognizes phosphorylated Rb (Ser807/Ser811) and does not react with non-phosphorylated Rb. Does not react with Rb phosphorylated at other sites.

Preparation Note

Purified using protein A and peptide affinity chromatography.

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RIDADR  NONH for all modes of transport
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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