EMAIL THIS PAGE TO A FRIEND
R6504 Sigma

RPMI-1640 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Purchase

Properties

Related Categories Cell Culture, Classic Media and Salts, Powder Media, RPMI- 1640 Medium
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   phenol red: yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
HEPES: no
storage temp.   2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

RPMI-1640 Medium is used for the following applications:
• used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)
• used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
• used for cell isolation and culture
• used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay
• used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
• used for the preparation of antifungal agents

Quantity

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

General description

Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


All labs need water

Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

Testing for Mycoplasma by Indirect DNA Stain

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction

Related Content

RPMI Media - RPMI-1640 Media

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system...
Keywords: Cell culture, Metabolism, Vitamins

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

supplement

Product #

Description

Add to Cart

F2442 Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
S5761 Sodium bicarbonate, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?