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R6525 Sigma

Anti-phospho-Rsk1 (p90rsk) (pSer381) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-phospho-Rsk 1 (pSer381)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphoserine (pSer),
species reactivity   mouse, rat, human
application(s)   immunocytochemistry: 1:2,000
  immunoprecipitation: 1:200
  western blot (chemiluminescent): 1:1,000 using TPA-treated 293 cell extract
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... RPS6KA1(6195)
mouse ... Rps6ka1(20111)
rat ... Rps6ka1(81771)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Synthetic phospho-Ser381 peptide corresponding to residues around Ser381 of human (p90rsk) conjugated to KLH.

General description

Rsk1 is a ubiquitously expressed, mitogen-activated protein that belongs to the serine/threonine protein kinase family. It has a pivotal role in facilitating MEK-MAP kinase cell survival signaling and supressing pro-apoptotic function of BAD by its phosphorylation. Anti-phospho-Rsk1 (p90rsk) (pSer381) antibody can be used to detect Rsk1 when phosphorylated at phosphoserine 381. It can also be used in immunoprecipitation (diluted 1:200). Rabbit anti-phospho-Rsk1 (p90rsk) (pSer381) antibody reacts specifically with phosphorylated Rsk1 and not with nonphosphorylated Rsk1. The product has also specificity for Rsk1 of rat, mouse and human.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

Application

Anti-phospho-Rsk1 (p90rsk) (pSer381) antibody can be used in western blotting (diluted 1:1000) using TPA-treated 293 cell extract. It can also be used in immunocytochemistry (diluted 1:2000).

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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