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R6775 Sigma

Anti-Retinoblastoma antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Rb

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using human colon adenocarcinoma tissue
  microarray: suitable
  western blot: 1:100 using human acute lymphoma Jurkat cell lysate
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 105 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... RB1(5925)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 914-928 of the C-terminal region of human p105 retinoblastoma protein (p105 Rb) with N-terminally added cysteine, conjugated to maleimide-activated KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Specificity

This sequence recognized by the antibody is highly conserved across species (e.g., mouse, rat p105 Rb) and is not found in p107 Rb and p130 Rb.

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