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R7020 Sigma

Transcript RNA Markers 0.2-10 kb

for RNA electrophoresis

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Properties

Related Categories Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology, Molecular Weight Marker,
grade   for molecular biology
form   liquid
usage   25 uses
suitability   suitable for electrophoresis (RNA)
storage temp.   −70°C

Description

Components

Sigma’s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

General description

Sigma’s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Other Notes

Contains nine defined RNA transcripts, 200–10,000 nucleotides

For optimal resolution, the recommended agarose gel concentration is 1.0%.

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3265 8 / PGIII
WGK Germany 
2
Protocols & Articles

Articles

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

Add to Cart

R7644 Transcript RNA Markers 0.28-6.6 kb, for RNA electrophoresis
R4268 RNA Sample Loading Buffer, for NA electrophoresis, with ethidium bromide (50 μg/mL)

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