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R7023 Sigma

Pst I from Providencia stuartii

Restriction Enzyme

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Description

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Comment: Pst I requires optimal reaction conditions in order to avoid star activity.

Specificity

Recognition sequence: 5′-CTGCA/G-3′
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Physical form

Solution in 10 mM Tris-HCl, pH 7.0, 1 mM EDTA, 250 mM NaCl, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4 °C

Application

PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Restriction Enzyme Buffer Reference

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer ...
Keywords: Buffers, Digestions, Enzyme activity, PAGE, Solid phase extractions

Restriction Enzyme Double Digest Buffer Guide

Sigma Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When using two restriction enzymes at once, first check the enzyme activities in each bu...
Keywords: Buffers, Digestions, Solid phase extractions

Protocols

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Restriction Enzyme Digest Protocol

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also...
Keywords: Buffers, Digestions, Purification

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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