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R7509 Sigma

RPMI-1640 Medium

Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Classic Media and Salts, RPMI- 1640 Medium More...
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   NaHCO3: yes
phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
storage temp.   2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities.
Use this medium when working with stem cells or when growing cells at low densities.
Also recommended for in vitro diagnostics use.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Cell Viability and Proliferation

Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an ass...
Mark Frei
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Flow cytometry, Mutagens, Reductions, Solvents

Related Content

RPMI Media - RPMI-1640 Media

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system...
Keywords: Cell culture, Metabolism, Vitamins

Peer-Reviewed Papers
15

References

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