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R7757 Sigma

Red Blood Cell Lysing Buffer Hybri-Max

liquid, sterile-filtered, suitable for hybridoma

Synonym: RBC Lysing Buffer

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Properties

Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Hybridoma Platform, Hybridoma Reagents, Reagents and Supplements,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for hybridoma

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Contains 8.3 g/L ammonium chloride in 0.01 M Tris-HCl buffer.

Application

Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.

Biochem/physiol Actions

Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.

Other Notes

Note: This product is intended for the removal of red blood cells from mice. This product may not be appropriate for the lysis of red blood cells of other animals. The suitability of the product in any application other than mouse splenocytes must be determined by the researcher.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Filter out uncertainty.

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles
Peer-Reviewed Papers
15

References

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