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R7761 Sigma

Monoclonal Anti-Rat Lambda Light Chains antibody produced in mouse

clone RL-6, ascites fluid

Synonym: Monoclonal Anti-Rat Lambda Light Chains

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX, Rat Secondary Antibodies and Conjugates,
biological source   mouse
antibody form   ascites fluid
clone   RL-6, monoclonal
contains   15 mM sodium azide
application(s)   capture ELISA: suitable
  dot blot: suitable
  immunocytochemistry: suitable
  indirect ELISA: 1:2,000
  western blot: suitable
isotype   IgG2a
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Specificity

The antibody reacts with an epitope on the rat λ light chain found in various rat immunoglobulin classes and subclsasses. No cross-reactivity is observed with rat κ chains or with IgG from human, mouse, bovine, rabbit, goat, sheep, horse, dog, chicken, pig or cat. Weak cross-reactivity is observed with guinea pig IgG.

Biochem/physiol Actions

Immunoglobulins (Ig) are produced by the B cells and play crucial role in host humoral immune response. A typical immunoglobulin consists of two heavy chains and two light chains. The two light chains are either κ or the λ chain. The B cells express one type of light chain during their life time.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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