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R7876 Sigma

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 unit/mg solid

Synonym: Transfer RNA, tRNA

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Properties

Quality Level   PREMIUM
grade   for molecular biology
type   Type V
assay   15-19 unit/mg solid
form   lyophilized powder
storage condition   desiccated
solubility   H2O: ~2 mg/mL, clear
storage temp.   2-8°C

Description

Components

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

General description

Wheat germ tRNA contains many different specific tRNA molecules.

Packaging

2500, 10000 units in glass bottle

500 units in poly bottle

Unit Definition

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Application

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.

Suitable for use as a carrier in nucleic acid purification and precipitation.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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