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R8506 Sigma

Not I from Nocardia otidiscaviarum

Restriction Enzyme

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Description

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Specificity

Recognition sequence: 5′-GC/GGCCGC-3′
Cutting results: a 2-10-fold Not I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4 °C.

Application

NotI is used in molecular biology methods as a DNA restriction enzyme that cuts DNA at the recognition site 5′-GC/GGCCGC-3′ to generate DNA fragments with 5′-cohesive ends.

Price and Availability


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Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Restriction Enzyme Buffer Reference

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer ...
Keywords: Buffers, Digestions, Enzyme activity, PAGE, Solid phase extractions

Restriction Enzyme Double Digest Buffer Guide

Sigma Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When using two restriction enzymes at once, first check the enzyme activities in each bu...
Keywords: Buffers, Digestions, Solid phase extractions

Protocols

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Restriction Enzyme Digest Protocol

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also...
Keywords: Buffers, Digestions, Purification

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Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.

Find enzymes / proteins, substrates, activators, and inhibitors. Resources for metabolic pathways, kinases, proteolytic enzymes and inhibitors, carbohydrate analysis as well as new cell signaling, an...
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Peer-Reviewed Papers

References

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Increased expression of CD27 on activated human memory B cells correlates with their commitment to the plasma cell lineage. Avery, D.T., et al. J. Immunol. 174, 4034, (2005)

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Epidemiological study of E. coli O157:H7 isolated in Northern Ireland using pulse-field gel electrophoresis (PFGE). Watabe, M., et al. Ulster Med. J. 77, 168, (2008)

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Highlights of the DNA cutters: a short history of the restriction enzymes. Loenen WA, Dryden DT, Raleigh EA, et al. Nucleic Acids Res. 42(1), 3-19, (2014)

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Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells. Zhu H, Lau CH, Goh SL, et al. Nucleic Acids Res. 41(19), e180, (2013)

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Single-cell imaging of HIV-1 provirus (SCIP). Di Primio C, Quercioli V, Allouch A, et al. Proc. Natl. Acad. Sci. U. S. A. 110(14), 5636-41, (2013)

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Organization of the BcgI restriction-modification protein for the cleavage of eight phosphodiester bonds in DNA. Smith RM, Marshall JJ, Jacklin AJ, et al. Nucleic Acids Res. 41(1), 391-404, (2013)

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Evaluation of vitamin D receptor (VDR) gene polymorphisms (FokI, TaqI and ApaI) in a family with dentinogenesis imperfecta. Ulucan K, Akyüz S, Ozbay G, et al. Tsitol. Genet. 47(5), 28-32, (2013)

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Improved vectors for selection of transgenic Caenorhabditis elegans. Ferguson AA, Cai L, Kashyap L, et al. Methods Mol. Biol. 940, 87-102, (2013)

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Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs. Mitro N, Gilardi F, Giudici M, et al. Methods Mol. Biol. 952, 137-44, (2013)

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Specific elimination of mutant mitochondrial genomes in patient-derived cells by mitoTALENs. Bacman SR, Williams SL, Pinto M, et al. Nat. Med. 19(9), 1111-3, (2013)

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E-TALEN: a web tool to design TALENs for genome engineering. Heigwer F, Kerr G, Walther N, et al. Nucleic Acids Res. 41(20), e190, (2013)

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Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats. Gabsalilow L, Schierling B, Friedhoff P, et al. Nucleic Acids Res. 41(7), e83, (2013)

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Using defined finger-finger interfaces as units of assembly for constructing zinc-finger nucleases. Zhu C, Gupta A, Hall VL, et al. Nucleic Acids Res. 41(4), 2455-65, (2013)

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Evaluating the mutagenic activity of targeted endonucleases containing a Sharkey FokI cleavage domain variant in zebrafish. Pillay LM, Selland LG, Fleisch VC, et al. Zebrafish 10(3), 353-64, (2013)

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Restriction enzymes and their isoschizomers. Roberts, R.J. Nucleic Acids Res. 18, 2331-2365, (1990)

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Double-strand break repair by homologous recombination in primary mouse somatic cells requires BRCA1 but not the ATM kinase. Kass EM, Helgadottir HR, Chen CC, et al. Proc. Natl. Acad. Sci. U. S. A. 110(14), 5564-9, (2013)

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Helicobacter pylori DprA alleviates restriction barrier for incoming DNA. Dwivedi GR, Sharma E, and Rao DN Nucleic Acids Res. 41(5), 3274-88, (2013)

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Palindromic sequence impedes sequencing-by-ligation mechanism. Huang YF, Chen SC, Chiang YS, et al. BMC Syst. Biol. 6 Suppl 2, S10, (2012)

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Conserved DNA methylation patterns in healthy blood cells and extensive changes in leukemia measured by a new quantitative technique. Jelinek J, Liang S, Lu Y, et al. Epigenetics 7(12), 1368-78, (2012)

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rDNA-directed integration by an HIV-1 integrase--I-PpoI fusion protein. Schenkwein D, Turkki V, Ahlroth MK, et al. Nucleic Acids Res. 41(5), e61, (2013)

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Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization. Lyumkis D, Talley H, Stewart A, et al. Structure 21(10), 1848-58, (2013)

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Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM. Finney-Manchester SP and Maheshri N Nucleic Acids Res. 41(9), e99, (2013)

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The MASTER (methylation-assisted tailorable ends rational) ligation method for seamless DNA assembly. Chen WH, Qin ZJ, Wang J, et al. Nucleic Acids Res. 41(8), e93, (2013)

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Transient systemic mtDNA damage leads to muscle wasting by reducing the satellite cell pool. Wang X, Pickrell AM, Rossi SG, et al. Hum. Mol. Genet. 22(19), 3976-86, (2013)

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IgH class switching exploits a general property of two DNA breaks to be joined in cis over long chromosomal distances. Gostissa M, Schwer B, Chang A, et al. Proc. Natl. Acad. Sci. U. S. A. 111(7), 2644-9, (2014)

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Increasing cleavage specificity and activity of restriction endonuclease KpnI. Vasu K, Nagamalleswari E, Zahran M, et al. Nucleic Acids Res. 41(21), 9812-24, (2013)

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Base-displaced intercalation of the 2-amino-3-methylimidazo[4,5-f]quinolone N2-dG adduct in the NarI DNA recognition sequence. Stavros KM, Hawkins EK, Rizzo CJ, et al. Nucleic Acids Res. 42(5), 3450-63, (2014)

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The association between BsmI polymorphism and bone mineral density in young patients with epilepsy who are taking phenytoin. Phabphal K, Geater A, Limapichart K, et al. Epilepsia 54(2), 249-55, (2013)

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HindII and SduI digests of heat-shock protein 70 PCR for Leishmania typing. Fraga J, Montalvo AM, Maes L, et al. Diagn. Microbiol. Infect. Dis. 77(3), 245-7, (2013)

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Genomic comparison of Salmonella typhimurium DT104 with non-DT104 strains. Zhao EY, Bao HX, Tang L, et al. Mol. Genet. Genomics 288(11), 549-57, (2013)

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CYP2E1 RsaI/PstI polymorphism and gastric cancer susceptibility: meta-analyses based on 24 case-control studies. Zhuo W, Zhang L, Wang Y, et al. PLoS ONE 7(11), e48265, (2012)

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Relationship of BsmI polymorphism of vitamin D receptor gene with left ventricular hypertrophy and atherosclerosis in hemodialysis patients. El-Shehaby AM, El-Khatib MM, Marzouk S, et al. Scand. J. Clin. Lab. Invest. 73(1), 75-81, (2013)

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Lack of association between vitamin D receptor gene FokI and BsmI polymorphisms and prostate cancer risk: an updated meta-analysis involving 21,756 subjects. Guo Z, Wen J, Kan Q, et al. Tumour Biol. 34(5), 3189-200, (2013)

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Associations between vitamin D receptor polymorphisms and breast cancer risk. Wang J, He Q, Shao YG, et al. Tumour Biol. 34(6), 3823-30, (2013)

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Interactions between serum vitamin D levels and vitamin D receptor gene FokI polymorphisms for renal function in patients with type 2 diabetes. Yokoyama K, Nakashima A, Urashima M, et al. PLoS ONE 7(12), e51171, (2012)

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Restriction endonuclease TseI cleaves A:A and T:T mismatches in CAG and CTG repeats. Ma L, Chen K, Clarke DJ, et al. Nucleic Acids Res. 41(9), 4999-5009, (2013)

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Gγ-Xmn I polymorphism: a significant determinant of β-thalassemia treatment without blood transfusion. Ansari SH, Shamsi TS, Munzir S, et al. J. Pediatr. Hematol. Oncol. 35(4), e153-6, (2013)

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The VDR gene FokI polymorphism and ovarian cancer risk. Xu H, Li S, Qiu JQ, et al. Tumour Biol. 34(6), 3309-16, (2013)

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Meta-analysis of the relation between vitamin D receptor gene BsmI polymorphism and susceptibility to ovarian cancer. Zhang Y, Tong SC, Guan LH, et al. Tumour Biol. 34(6), 3317-21, (2013)

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Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V. Baumann T, Arndt KM, and Müller KM BMC Biotechnol. 13, 81, (2013)

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CYP1AI and CYP2E1 gene polymorphisms may increase susceptibility to Oral Submucous Fibrosis among betel quid chewers of Eastern India. Chaudhuri SR, Mukherjee S, Paul RR, et al. Gene 513(2), 268-71, (2013)

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Interleukin-6 promoter polymorphisms -174 G/C in Brazilian patients with systemic lupus erythematosus. Asano NM, Angelo HD, da Silva HA, et al. Hum. Immunol. 74(9), 1153-6, (2013)

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Inter-batch contamination and potential sources of vancomycin-resistant Enterococcus faecium on broiler farms. Jansson DS, Nilsson O, Lindblad J, et al. Br. Poult. Sci. 53(6), 790-9, (2012)

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Using single-turnover kinetics with osmotic stress to characterize the EcoRV cleavage reaction. Ferrandino R, Sidorova N, and Rau D Biochemistry 53(1), 235-46, (2014)

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Effects of vitamin D receptor polymorphisms on urolithiasis risk: a meta-analysis. Zhang P, Nie W, and Jiang H BMC Med. Genet. 14, 104, (2013)

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Dopamine D2 receptor and ankyrin repeat and kinase domain containing 1 genes among the two linguistically distinct tribal populations of Ranchi district, Jharkhand, India. Sachdeva MP, Saksena D, Mondal PR, et al. Anthropol. Anz. 70(1), 15-26, (2013)

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A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases. Podgórska B, Kujawska G, Skurzewski M, et al. Acta Biochim. Pol. 59(4), 669-72, (2012)

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Serum vitamin D levels and VDR polymorphisms (BsmI and FokI) in patients and their household contacts susceptible to tuberculosis. Joshi L, Ponnana M, Penmetsa SR, et al. Scand. J. Immunol. 79(2), 113-9, (2014)

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Association of the estrogen receptor-β gene RsaI and AluI polymorphisms with human idiopathic thin endometrium. Le AW, Wang ZH, Yuan R, et al. Genet. Mol. Res. 12(4), 5978-85, (2013)

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[Utility of chromosome banding with ALU I enzyme for identifying methylated areas in breast cancer]. Rojas-Atencio A, Yamarte L, Urdaneta K, et al. Invest. Clin. 53(4), 331-41, (2012)

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The architecture of restriction enzymes. Dryden DT Structure 21(10), 1720-1, (2013)

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Tailor-made mutations in Arabidopsis using zinc finger nucleases. Qi Y, Starker CG, Zhang F, et al. Methods Mol. Biol. 1062, 193-209, (2014)

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TALENs and ZFNs are associated with different mutation signatures. Kim Y, Kweon J, and Kim JS Nature Methods 10(3), 185, (2013)

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