Not I from Nocardia otidiscaviarum
buffered aqueous glycerol solution
DOWNLOAD MSDS (PDF)-
CAS Number 103780-20-7
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MDL number MFCD00165010
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
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Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Core Bioreagents, |
| form | buffered aqueous glycerol solution |
| concentration | 10,000 units/mL |
| shipped in | wet ice |
| storage temp. | −20°C |
Description
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Specificity
Recognition sequence: 5′-GC/GGCCGC-3′
Ligation and recutting results: After 2-10-fold Not I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and 95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4 °C.
Application
NotI is used in molecular biology methods as a DNA restriction enzyme that cuts DNA at the recognition site 5′-GC/GGCCGC-3′ to generate DNA fragments with 5′-cohesive ends.
Price and Availability
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buffered aqueous glycerol solution
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