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R8506 Sigma

Not I from Nocardia otidiscaviarum

Restriction Enzyme


Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).


Recognition sequence: 5′-GC/GGCCGC-3′
Cutting results: a 2-10-fold Not I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4 °C.


NotI is used in molecular biology methods as a DNA restriction enzyme that cuts DNA at the recognition site 5′-GC/GGCCGC-3′ to generate DNA fragments with 5′-cohesive ends.

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Oxford Genetics

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