EMAIL THIS PAGE TO A FRIEND
R8755 Sigma

RPMI-1640 Medium

Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Purchase

Properties

Related Categories Cell Culture, Classic Media and Salts, Powder Media, RPMI- 1640 Medium
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
HEPES: no
phenol red: no
storage temp.   2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

RPMI-1640 Medium is used for the following applications:
• Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
• Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
• Used for cell isolation and culture
• Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
• Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
• Used for the preparation of antifungal agents

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.

Quantity

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability


Stericup and Steritop Filter Units
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Cell Viability and Proliferation

Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an ass...
Mark Frei
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Flow cytometry, Mutagens, Reductions, Solvents

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

RPMI Media - RPMI-1640 Media

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system...
Keywords: Cell culture, Metabolism, Vitamins

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

supplement

Product #

Description

Add to Cart

F2442 Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
P3532 Phenol Red, powder, BioReagent, suitable for cell culture
S5761 Sodium bicarbonate, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?