Technical information & documentation associated with this product is available in the
Safety & Documentation tab.
Articles
The MISSION® Target ID Library for Human miRNA Target Identification and Discovery
One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are...
Keywords: AGE, Amplification, Apoptosis, Cancer, Cell culture, Cloning, Degradations, Detection methods, Digestions, Electrophoresis, Environmental, Fractionation, Gel electrophoresis, Gene expression, Genomics, Immunoprecipitation, Melting, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA immunoprecipitation, Reductions, Sequencing, Transfection, Western blot, transformation
Protocols
Cell Quantification
For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation
Cryopreservation of Cell Lines
The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions
Resuscitation of Frozen Cell Lines
Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE
Subculture of Adherent Cell Lines
Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion
Subculture of Semi-Adherent Cell Lines
Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture
Subculture of Suspension Cell Lines
In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors
Testing for Mycoplasma by Indirect DNA Stain
DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction
Papers
Set your institution to view full text papers.
Culture of normal human leukocytes Moore GE, et al. J. Am. Med. Assoc. 199, 519-524, (1967)
Loading...
Partial substitution of serum in hematopoietic cell line media by synthetic polymers Mizrahi A, and Moore GE. Appl. Microbiol. 19, 906-910, (1970)
Loading...
PI3K signaling of autophagy is required for starvation tolerance and virulenceof Cryptococcus neoformans. Guowu Hu et al J. Clin. Invest. 118, 1186-97, (2008)
Loading...
Distinct cell-specific control of autoimmunity and infection by FcγRIIb. Rebecca J. Brownlie et al J. Exp. Med. 205, 883-95, (2008)
Loading...
Improved vascular engraftment and graft function after inhibition of the angiostatic factor thrombospondin-1 in mouse pancreatic islets. Johan Olerud et al Diabetes 57, 1870-7, (2008)
Loading...
G/T substitution in intron 1 of the UNC13B gene is associated with increased risk of nephropathy in patients with type 1 diabetes. David-Alexandre Trégouet et al Diabetes 57, 2843-50, (2008)
Loading...
GRIM-19 is essential for maintenance of mitochondrial membrane potential. Hao Lu et al Mol. Biol. Cell 19, 1893-902, (2008)
Loading...
Natural selection of FLT1 alleles and their association with malaria resistance in utero. Atis Muehlenbachs et al Proc. Natl. Acad. Sci. U. S. A. 105, 14488-91, (2008)
Loading...
Low-dose priming before vaccination with the phase I chloroform-methanol residue vaccine against Q fever enhances humoral and cellular immune responses to Coxiella burnetii. David M. Waag et al Clin. Vaccine Immunol. 15, 1505-12, (2008)
Loading...
Comparison of assessment of oxygen consumption, Etest, and CLSI M38-A2 broth microdilution methods for evaluation of the susceptibility of Aspergillus fumigatus to posaconazole. Ricardo Araujo et al Antimicrob. Agents Chemother. 53, 4921-3, (2009)
Loading...
CXCL8/IL-8 and CXCL12/SDF-1α co-operatively promote invasiveness and angiogenesis in pancreatic cancer. Yoichi Matsuo et al Int. J. Cancer 124, 853-61, (2009)
Loading...
Toll-like receptor 9 activation is a key mechanism for the maintenance of chronic lung inflammation. Toshihiro Ito et al Am. J. Respir. Crit. Care Med. 180, 1227-38, (2009)
Loading...
The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio. T.M. Suszynski et al Transplant. Proc. 40, 346-50, (2009)
Loading...
A Drosophila model identifies calpains as modulators of the human leukemogenic fusion protein AML1-ETO. Dani Osman et al Proc. Natl. Acad. Sci. U. S. A. 106, 12043-8, (2009)
Loading...
Protein kinase Cδ stimulates proteasome-dependent degradation of C/EBPα during apoptosis induction of leukemic cells. Meng Zhao et al PLoS ONE 4, e6552, (2009)
Loading...
Impairment of γ interferon signaling in human neutrophils infected with Anaplasma phagocytophilum. Uta Bussmeyer et al Infect. Immun. 78, 358-63, (2010)
Loading...
Donepezil, anti-Alzheimer's disease drug, prevents cardiac rupture during acute phase of myocardial infarction in mice. Mikihiko Arikawa et al PLoS ONE 6, e20629, (2011)
Loading...
Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Wong Yau Hsiung et al Evid. Based. Complement. Alternat. Med. 2011, 293060, (2011)
Loading...
Nitric Oxide (NO) and Cyclooxygenase-2 (COX-2) Cross-Talk in Co-Cultures of Tumor Spheroids with Normal Cells. Roman Paduch et al Cancer Microenviron. 4, 187-98, (2011)
Loading...
Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis. Caroline Le Maréchal et al Vet. Res. 42, 35, (2011)
Loading...
Identification of an unique CXCR4 epitope whose ligation inhibits infection by both CXCR4 and CCR5 tropic human immunodeficiency type-I viruses. Adachi T Retrovirology 8, 84, (2011)
Loading...
Polysaccharides from Agaricus bisporus and Agaricus brasiliensis show similarities in their structures and their immunomodulatory effects on human monocytic THP-1 cells. Smiderle FR BMC Complement Altern. Med. 11, 58, (2011)
Loading...
High-level fluorescence labeling of gram-positive pathogens. Aymanns S PLoS ONE 6(6), e19822, (2011)
Loading...
Peptidoglycan from lactobacilli inhibits interleukin-12 production by macrophages induced by Lactobacillus casei through Toll-like receptor 2-dependent and independent mechanisms. Shida K Immunology 128(1 Suppl), e858-69, (2009)
Loading...
Up-regulation of Th17 cells may underlie inhibition of Treg development caused by immunization with activated syngeneic T cells. Wang L PLoS ONE 6(11), e27289, (2011)
Loading...
In situ prior proliferation of CD4+ CCR6+ regulatory T cells facilitated by TGF-β secreting DCs is crucial for their enrichment and suppression in tumor immunity. Xu L PLoS ONE 6(5), e20282, (2011)
Loading...
The heterogeneous ribonuclear protein C interacts with the hepatitis delta virus small antigen. Casaca A Virol. J. 8, 358, (2011)
Loading...
Fungal recognition enhances mannose receptor shedding through dectin-1 engagement. Gazi U J. Biol. Chem. 286(10), 7822-9, (2011)
Loading...
A distinct peripheral blood monocyte phenotype is associated with parasite inhibitory activity in acute uncomplicated Plasmodium falciparum malaria. Chimma P PLoS Pathog. 5(10), e1000631, (2009)
Loading...