EMAIL THIS PAGE TO A FRIEND

R8758 Sigma

RPMI-1640 Medium

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Purchase

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Protocols & Applications

Aseptic Techniques Video

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability

Timestrip - Time & Temperature Indicators


Timestrip
Discover how Timestrip® indicators will help you monitor expiry dates or excessive temperatures.





Request a Serum Sample

Additional Products to Explore

With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Penicillin-<WBR>Streptomycin

Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, sterile-filtered, BioReagent, suitable for cell culture

Customers Also Viewed

With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture

Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

with 2.05 mM L-glutamine, with 25mM HEPES, liquid, sterile-filtered, suitable for cell culture

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Safety & Documentation

Safety Information

WGK Germany 
1

Protocols & Articles

Articles

The MISSION® Target ID Library for Human miRNA Target Identification and Discovery

One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are...
Keywords: AGE, Amplification, Apoptosis, Cancer, Cell culture, Cloning, Degradations, Detection methods, Digestions, Electrophoresis, Environmental, Fractionation, Gel electrophoresis, Gene expression, Genomics, Immunoprecipitation, Melting, Methods, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA immunoprecipitation, Reductions, Sequences, Sequencing, Support, Transfection, Western blot, transformation

Protocols

Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

Testing for Mycoplasma by Indirect DNA Stain

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction

Related Content

RPMI Media - RPMI-1640 Media

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system...
Keywords: Cell culture, Metabolism, Vitamins

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage. Arita R Diabetes 58(1), 215-26, (2009)

Loading...


Distinct cell-specific control of autoimmunity and infection by FcγRIIb. Rebecca J. Brownlie et al J. Exp. Med. 205, 883-95, (2008)

Loading...


Improved vascular engraftment and graft function after inhibition of the angiostatic factor thrombospondin-1 in mouse pancreatic islets. Johan Olerud et al Diabetes 57, 1870-7, (2008)

Loading...


Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis. Caroline Le Maréchal et al Vet. Res. 42, 35, (2011)

Loading...


Polysaccharides from Agaricus bisporus and Agaricus brasiliensis show similarities in their structures and their immunomodulatory effects on human monocytic THP-1 cells. Smiderle FR BMC Complement Altern. Med. 11, 58, (2011)

Loading...


Surface-engineered targeted PPI dendrimer for efficient intracellular and intratumoral siRNA delivery. Taratula O J. Control. Release 140(3), 284-93, (2009)

Loading...


Aberrant hyperactivation of akt and Mammalian target of rapamycin complex 1 signaling in sporadic chordomas. Han S Clin. Cancer Res. 15(6), 1940-6, (2009)

Loading...


The MyD88+ phenotype is an adverse prognostic factor in epithelial ovarian cancer. d'Adhemar CJ, Spillane CD, Gallagher MF, et al. PLoS ONE 9(6), e100816, (2014)

Loading...


G/T substitution in intron 1 of the UNC13B gene is associated with increased risk of nephropathy in patients with type 1 diabetes. David-Alexandre Trégouet et al Diabetes 57, 2843-50, (2008)

Loading...


Impairment of γ interferon signaling in human neutrophils infected with Anaplasma phagocytophilum. Uta Bussmeyer et al Infect. Immun. 78, 358-63, (2010)

Loading...


Toll-like receptor 9 activation is a key mechanism for the maintenance of chronic lung inflammation. Toshihiro Ito et al Am. J. Respir. Crit. Care Med. 180, 1227-38, (2009)

Loading...


MAPK kinase kinase-1 is essential for cytokine-induced c-Jun NH2-terminal kinase and nuclear factor-kappaB activation in human pancreatic islet cells. Mokhtari D Diabetes 57(7), 1896-904, (2008)

Loading...


PI3K signaling of autophagy is required for starvation tolerance and virulenceof Cryptococcus neoformans. Guowu Hu et al J. Clin. Invest. 118, 1186-97, (2008)

Loading...


Nonhematopoietic cells control the outcome of infection with Listeria monocytogenes in a nucleotide oligomerization domain 1-dependent manner. Mosa A Infect. Immun. 77(7), 2908-18, (2009)

Loading...


Natural selection of FLT1 alleles and their association with malaria resistance in utero. Atis Muehlenbachs et al Proc. Natl. Acad. Sci. U. S. A. 105, 14488-91, (2008)

Loading...


Genetic analysis of the cytoplasmic domain of the PfRh2b merozoite invasion protein of Plasmodium falciparum. DeSimone TM Int. J. Parasitol. 39(4), 399-405, (2009)

Loading...


Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults. Kekkonen, RA World J. Gastroenterol. 14(13), 2029-36, (2008)

Loading...


Nitric Oxide (NO) and Cyclooxygenase-2 (COX-2) Cross-Talk in Co-Cultures of Tumor Spheroids with Normal Cells. Roman Paduch et al Cancer Microenviron. 4, 187-98, (2011)

Loading...


Safety and Immunogenicity of a Human Papillomavirus Peptide Vaccine (CIGB-228) in Women with High-Grade Cervical Intraepithelial Neoplasia: First-in-Human, Proof-of-Concept Trial. Solares AM, Baladron I, Ramos T, et al. ISRN Obstet. Gynecol. 2011, 292951, (2011)

Loading...


Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells. Wong Yau Hsiung et al Evid. Based. Complement. Alternat. Med. 2011, 293060, (2011)

Loading...


Randomized phase II clinical trial of chemo-immunotherapy in advanced nonsmall cell lung cancer. Lasalvia-Prisco E Biologics 2(3), 555-61, (2008)

Loading...


Hierarchical clustering of monoclonal antibody reactivity patterns in nonhuman species. Juan Pablo Pratt et al Cytometry. A 75, 734-42, (2010)

Loading...


Fungal recognition enhances mannose receptor shedding through dectin-1 engagement. Gazi U J. Biol. Chem. 286(10), 7822-9, (2011)

Loading...


A sterol-regulatory element binding protein is required for cell polarity, hypoxia adaptation, azole drug resistance, and virulence in Aspergillus fumigatus. Willger SD PLoS Pathog. 4(11), e1000200, (2008)

Loading...


The Sur7 protein regulates plasma membrane organization and prevents intracellular cell wall growth in Candida albicans. Alvarez FJ Mol. Biol. Cell 19(12), 5214-25, (2008)

Loading...


The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio. T.M. Suszynski et al Transplant. Proc. 40, 346-50, (2009)

Loading...


Identification of an unique CXCR4 epitope whose ligation inhibits infection by both CXCR4 and CCR5 tropic human immunodeficiency type-I viruses. Adachi T Retrovirology 8, 84, (2011)

Loading...


A dominant role for the immunoproteasome in CD8+ T cell responses to murine cytomegalovirus. Hutchinson S, Sims S, O'Hara G, et al. PLoS ONE 6(2), e14646, (2011)

Loading...


Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts. Pilling D PLoS ONE 4(10), e7475, (2009)

Loading...


Genotyping of Trypanosoma cruzi: systematic selection of assays allowing rapid and accurate discrimination of all known lineages. Lewis MD Am. J. Trop. Med. Hyg. 81(6), 1041-9, (2009)

Loading...


CXCL8/IL-8 and CXCL12/SDF-1α co-operatively promote invasiveness and angiogenesis in pancreatic cancer. Yoichi Matsuo et al Int. J. Cancer 124, 853-61, (2009)

Loading...


Lack of association of TIM3 polymorphisms and allergic phenotypes. Zhang, J BMC Med. Genet. 10, 62, (2009)

Loading...


IL11 antagonist inhibits uterine stromal differentiation, causing pregnancy failure in mice. Menkhorst E Biol. Reprod. 80(5), 920-7, (2009)

Loading...


Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR) and concomitant activation of gelatinolytic enzymes. Magnussen S, Hadler-Olsen E, Latysheva N, et al. PLoS ONE 9(8), e105929, (2014)

Loading...


Chronic antidiabetic sulfonylureas in vivo: reversible effects on mouse pancreatic beta-cells. Remedi MS PLoS Med. 5(10), e206, (2008)

Loading...


Whole-exome sequencing of DNA from peripheral blood mononuclear cells (PBMC) and EBV-transformed lymphocytes from the same donor. Londin ER BMC Genomics 12, 464, (2011)

Loading...


Inhibition of oesophageal squamous cell carcinoma progression by in vivo targeting of hyaluronan synthesis. Twarock S, Freudenberger T, Poscher E, et al. Mol. Cancer 10, 30, (2011)

Loading...


Chloroquine synergizes sunitinib cytotoxicity via modulating autophagic, apoptotic and angiogenic machineries. Abdel-Aziz AK, Shouman S, El-Demerdash E, et al. Chem. Biol. Interact. 217, 28-40, (2014)

Loading...


Expression of BMP-7 in human gastric cancer and its clinical significance. Aoki M, Ishigami S, Uenosono Y, et al. Br. J. Cancer 104(4), 714-8, (2011)

Loading...


Rapid diagnosis of Mycobacterium tuberculosis meningitis by enumeration of cerebrospinal fluid antigen-specific T-cells. Thomas MM Int. J. Tuberc. Lung Dis. 12(6), 651-7, (2008)

Loading...


GRIM-19 is essential for maintenance of mitochondrial membrane potential. Hao Lu et al Mol. Biol. Cell 19, 1893-902, (2008)

Loading...


Serpin induced antiviral activity of prostaglandin synthetase-2 against HIV-1 replication. Whitney JB PLoS ONE 6(4), e18589, (2011)

Loading...


CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells. Pino SC PLoS ONE 4(5), e5468, (2009)

Loading...


Protein kinase C signaling during T cell activation induces the endoplasmic reticulum stress response. Pino SC Cell Stress Chaperones 13(4), 421-34, (2008)

Loading...


LPS regulates SOCS2 transcription in a type I interferon dependent autocrine-paracrine loop. Hu J, Lou D, Carow B, et al. PLoS ONE 7(1), e30166, (2012)

Loading...


Peptidoglycan from lactobacilli inhibits interleukin-12 production by macrophages induced by Lactobacillus casei through Toll-like receptor 2-dependent and independent mechanisms. Shida K Immunology 128(1 Suppl), e858-69, (2009)

Loading...


Leishmania donovani infection down-regulates TLR2-stimulated IL-12p40 and activates IL-10 in cells of macrophage/monocytic lineage by modulating MAPK pathways through a contact-dependent mechanism. Chandra D Clin. Exp. Immunol. 154(2), 224-34, (2008)

Loading...


Long-term exposure to genistein improves insulin secretory function of pancreatic beta-cells. Fu Z and Liu D Eur. J. Pharmacol. 616(1-3), 321-7, (2009)

Loading...


In infertile women, cells from Chlamydia trachomatis infected sites release higher levels of interferon-gamma, interleukin-10 and tumor necrosis factor-alpha upon heat-shock-protein stimulation than fertile women. Srivastava P Reprod. Biol. Endocrinol. 6, 20, (2008)

Loading...


Suppression of blastogenesis and proliferation of activated CD4(+) T cells: intravenous immunoglobulin (IVIg) versus novel anti-human leucocyte antigen (HLA)-E monoclonal antibodies mimicking HLA-I reactivity of IVIg. Ravindranath MH, Terasaki PI, Pham T, et al. Clin. Exp. Immunol. 178(1), 154-77, (2014)

Loading...


Enhanced release of IgE-dependent early phase mediators from nasal polyp tissue. Patou J, Holtappels G, Affleck K, et al. J. Inflamm. (Lond.) 6, 11, (2009)

Loading...


Immunization with oligomannose-coated liposome-entrapped dense granule protein 7 protects dams and offspring from Neospora caninum infection in mice. Nishikawa Y Clin. Vaccine Immunol. 16(6), 792-7, (2009)

Loading...


In situ prior proliferation of CD4+ CCR6+ regulatory T cells facilitated by TGF-β secreting DCs is crucial for their enrichment and suppression in tumor immunity. Xu L PLoS ONE 6(5), e20282, (2011)

Loading...


Response of macrophage Toll-like receptor 4 to a Sporothrix schenckii lipid extract during experimental sporotrichosis. Sassá MF Immunology 128(2), 301-9, (2009)

Loading...


Variant HNF1 modulates epithelial plasticity of normal and transformed ovary cells. Tomassetti A Neoplasia 10(12), 1481-92, 3p following 1492, (2008)

Loading...


Comparison of assessment of oxygen consumption, Etest, and CLSI M38-A2 broth microdilution methods for evaluation of the susceptibility of Aspergillus fumigatus to posaconazole. Ricardo Araujo et al Antimicrob. Agents Chemother. 53, 4921-3, (2009)

Loading...


Selenium enrichment of broccoli sprout extract increases chemosensitivity and apoptosis of LNCaP prostate cancer cells. Abdulah R BMC Cancer 9, 414, (2009)

Loading...


A Drosophila model identifies calpains as modulators of the human leukemogenic fusion protein AML1-ETO. Dani Osman et al Proc. Natl. Acad. Sci. U. S. A. 106, 12043-8, (2009)

Loading...


High-level fluorescence labeling of gram-positive pathogens. Aymanns S PLoS ONE 6(6), e19822, (2011)

Loading...


The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane. Liaunardy-Jopeace A, Bryant CE, and Gay NJ Sci. Signal. 7(336), ra70, (2014)

Loading...


Antitumor effect and mechanism of Gecko on human esophageal carcinoma cell lines in vitro and xenografted sarcoma 180 in Kunming mice. Liu F World J. Gastroenterol. 14(25), 3990-6, (2008)

Loading...


Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Zomorodian K, Saharkhiz MJ, Rahimi MJ, et al. Pharmacogn. Mag. 7(25), 53-9, (2011)

Loading...


Molecular characterization of chordoma xenografts generated from a novel primary chordoma cell source and two chordoma cell lines. Karikari IO, Gilchrist CL, Jing L, et al. J. Neurosurg. Spine 21(3), 386-93, (2014)

Loading...


Controlling viral immuno-inflammatory lesions by modulating aryl hydrocarbon receptor signaling. Veiga-Parga T, Suryawanshi A, and Rouse BT PLoS Pathog. 7(12), e1002427, (2011)

Loading...


A distinct peripheral blood monocyte phenotype is associated with parasite inhibitory activity in acute uncomplicated Plasmodium falciparum malaria. Chimma P PLoS Pathog. 5(10), e1000631, (2009)

Loading...


Up-regulation of Th17 cells may underlie inhibition of Treg development caused by immunization with activated syngeneic T cells. Wang, L PLoS ONE 6(11), e27289, (2011)

Loading...


Donepezil, anti-Alzheimer's disease drug, prevents cardiac rupture during acute phase of myocardial infarction in mice. Mikihiko Arikawa et al PLoS ONE 6, e20629, (2011)

Loading...


The heterogeneous ribonuclear protein C interacts with the hepatitis delta virus small antigen. Casaca A Virol. J. 8, 358, (2011)

Loading...


Hepatitis C virus NS3 protein can activate the Notch-signaling pathway through binding to a transcription factor, SRCAP. Iwai A, Takegami T, Shiozaki T, et al. PLoS ONE 6(6), e20718, (2011)

Loading...


Tumor-shed PGE(2) impairs IL2Rgammac-signaling to inhibit CD4 T cell survival: regulation by theaflavins. Chattopadhyay, S PLoS ONE 4(10), e7382, (2009)

Loading...


Low-dose priming before vaccination with the phase I chloroform-methanol residue vaccine against Q fever enhances humoral and cellular immune responses to Coxiella burnetii. David M. Waag et al Clin. Vaccine Immunol. 15, 1505-12, (2008)

Loading...


Activation of monocytes and cytokine production in patients with peripheral atherosclerosis obliterans. Corrêa CR, Dias-Melicio LA, Calvi SA, et al. J. Inflamm. (Lond.) 8, 23, (2011)

Loading...


DNA vaccines against dengue virus type 2 based on truncate envelope protein or its domain III. Azevedo AS, Yamamura AM, Freire MS, et al. PLoS ONE 6(7), e20528, (2011)

Loading...


The impact of Nucleofection® on the activation state of primary human CD4 T cells. Zhang M, Ma Z, Selliah N, et al. J. Immunol. Methods 408, 123-31, (2014)

Loading...


Effects of ferumoxides-protamine sulfate labeling on immunomodulatory characteristics of macrophage-like THP-1 cells. Janic B PLoS ONE 3(6), e2499, (2008)

Loading...


Protein kinase Cδ stimulates proteasome-dependent degradation of C/EBPα during apoptosis induction of leukemic cells. Meng Zhao et al PLoS ONE 4, e6552, (2009)

Loading...


Partial substitution of serum in hematopoietic cell line media by synthetic polymers Mizrahi A, and Moore GE. Appl. Microbiol. 19, 906-910, (1970)

Loading...


Culture of normal human leukocytes Moore GE, et al. J. Am. Med. Assoc. 199, 519-524, (1967)

Loading...


Related Products

also commonly purchased with this product

Product #

Description

Add to Cart

D8537 Dulbecco’s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
D5796 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
F1051 Fetal Bovine Serum, Canada origin, sterile-filtered, suitable for cell culture
CLS430769 Corning® bottle-top vacuum filter system, cellulose acetate membrane, pore size 0.22 μm, membrane area 33.2 cm2, filter capacity 500 mL

recommended

Product #

Description

Add to Cart

CLS430641 Corning® cell culture flasks, surface area 75 cm2, canted neck, cap (vented)

related product

Product #

Description

Add to Cart

F2442 Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?