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R9010 Sigma

Monoclonal Anti-Rat Kappa Light Chains (1a + 1b) antibody produced in mouse

clone RT-39, ascites fluid

Synonym: Monoclonal Anti-Rat Kappa Light Chains (1a + 1b)

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX, Rat Secondary Antibodies and Conjugates,
biological source   mouse
antibody form   ascites fluid
clone   RT-39, monoclonal
mol wt   antigen mol wt 25 kDa
contains   15 mM sodium azide
application(s)   capture ELISA: suitable
  dot blot: suitable
  immunocytochemistry: suitable
  indirect ELISA: 1:2,000
  western blot: suitable using denatured and reduced normal serum or myeloma protein
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified rat IgG

Specificity

The antibody reacts with an epitope on the rat κ light chain, which is shared by both the 1a and 1b allotypes in various rat immunoglobulin classes and subclasses. No cross-reactivity is observed with rat λ light chains or with IgG from human, mouse, bovine, rabbit, goat, sheep, horse, dog, chicken, pig or cat. The product shows weak cross-reaction with guinea pig IgG.

Application

Monoclonal Anti-Rat κ Light Chains (1a + 1b) antibody is suitable for use in capture ELISA. The product can also be used for dot blot and immunocytochemistry.

General description

Rat immunoglobulins are often used as primary antibodies against target proteins in several immunoassays. Thus, secondary anti-rat antibodies are useful tools for the detection and localization of target proteins. Moreover, the use of monoclonal secondary antibodies improves the accuracy of test results as they do not cross-react with immunoglobulins from other species. Monoclonal Anti-Rat κ Light Chains (1a + 1b) antibody is specific for an epitope on the κ light chain (1a and 1b) allotypes of all rat immunoglobulins. The antibody recognizes κ light chains in normal serum and myeloma proteins, but does not detect rat λ chains. The product reacts with the denatured-reduced molecule. The antibody weakly associates with guinea pig immunoglobulins but does not bind to human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit and sheep IgGs.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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