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R9255 Sigma

Anti-Rat IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX, Rat Secondary Antibodies and Conjugates,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
concentration   7-14 mg/mL (by absorbance at 280 nm)
application(s)   indirect ELISA: 1:100,000
  quantitative precipitin assay: 2.5-5 mg/mL
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Application

Anti-Rabbit IgG (whole molecule) antibody produced in rabbit was used in to precipitate and quantify the titer of human growth hormone and anti-human growth hormone complexes at a dilution of 1:20.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Protocols

Imprint RIP | uRNA Quantitation | MicroRNA primers | MicroRNA Reverse Transcription

Sigma’s Imprint RNA Immunoprecipitation Kit was used to co-purify human argonaute 2 (Ago2)-associated RNAs from HeLa cells, essentially as instructed in the Technical Bulletin. To evaluate the differ...
Carol Kreader
Principal R&D Scientist
Keywords: Buffers, Cell disruption, Immunoprecipitation, Polymerase chain reaction - quantitative, RNA immunoprecipitation, Western blot

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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