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R9376 Sigma

AMV-Reverse Transcriptase from avian myeloblastosis virus

Synonym: RNA-directed DNA polymerase, Revertase

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
concentration   10,000-100,000 units/mL
shipped in   dry ice
storage temp.   −70°C

Description

Application

AMV reverse transcriptase synthesizes DNA complementary (cDNA) to RNA templates. A DNA primer complementary to the RNA template and a divalent cation, either Mg or Mn, are required for initiation of transcription. This enzyme is commonly used to make cDNAs from mRNA for eventual cloning or for use as probes.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Unit Definition

One unit incorporates 1 nmol of dTTP into TCA precipitable material in 10 min at 37 °C using polyadenylic acid as template and oligo(dT)12-18 as a primer.

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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Peer-Reviewed Papers
15

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