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RAB0333 Sigma

Human Leptin ELISA Kit

for serum, plasma, cell culture supernatant and urine

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
application(s)   ELISA: suitable
  capture ELISA: suitable
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... LEP(3952)

Description

Application

Please refer to the attached Protocolfor details.

General description

The Human Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Leptin in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Leptin

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Price and Availability


Direct Detect Infrared Spectrometer

Kit component only

Description

Product #

Add to Cart

Human Leptin Antibody-coated ELISA Plate (Item A) SDS    
Lyophilized Human Leptin Protein Standard (Item C) SDS    
Biotinylated Human Leptin Detection Antibody (Item F) SDS    
ELISA 1X Assay/Sample Diluent Buffer A (Item D1) SDS    
ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS    
HRP-Streptavidin (Item G) SDS    
ELISA Stop Solution (Item I) SDS    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS    
20X Wash Buffer (Item B) SDS    
Safety & Documentation

Safety Information

RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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