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RECOMT Sigma

Thrombin CleanCleave Kit

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Description

Application

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Features and Benefits

• Fast, efficient cleavage in as little as 2 hours
• Thrombin is covalently bound to agarose for easy removal.
• The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
• Cleave tags even in the presence of 0.1% Triton, 1 M urea or 5 mM EDTA
• Thrombin-agarose is reusable.

Quantity

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

General description

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Legal Information

CleanCleave is a trademark of Sigma-Aldrich Co. LLC

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability


Amplified Detection

Standard component

Description

Product #

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10× Protein Cleavage Buffer 10 mL SDS T9685
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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