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S0189 Sigma

Stemline® Hematopoietic Stem Cell Expansion Medium

Serum-free, Without L-glutamine, liquid, sterile-filtered, suitable for cell culture




Intended for the expansion of CD 34+ progenitors derived from bone marrow, mobilized peripheral blood and cord blood.

Features and Benefits

Contains only human serum albumin and cholesterol derived from sheep wool and no other animal-derived components to facilitate its use in clinical investigations. Medium has been tested extensively in development in 7 day expansion assays with a starting inoculum of 10,000; and with HPP-CFC′s to demonstrate renewal of progenitors in CFU assays.

General description

Developed to promote the optimal expansion of human hematopoietic stem cells (HSC) from bone marrow, mobilized peripheral blood, and cord blood, Stemline Hematopoietic Stem Cell Expansion Medium demonstrates higher total nucleated cell (TNC) fold increases than other commercially available serum-free media formulations. Additionally, colony-forming unit (CFU) assays indicate that CD34+ hematopoietic stem cells grown in Stemline media demonstrate significantly greater expansion of bother early (HPP-CFC) and late (GM-CFC) progenitor populations when compared to those grown in alternative media. Human cord blood cells expanded in Stemline Media demonstrate impressive self-revewal when transplanted into immunodeficient NOC/SCID mice, illustrating Stemline′s utility in true functional trial.

Stemline Hematopoietic Stem Cell Expansion Medium free of serum and all other animal-derived components with the exception of human serum albumin and cholesterol derived from sheep wool. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.

Produced in a GMP state-of-the-art facility with an available Device Master File (DMF), Stemline Hematopoietic Stem Cell Expansion Medium is clearly an excellent choice for your HSC applications.

Other Notes

Proprietary formulation containing all non-animal derived components except human serum albumin and cholesterol derived from sheep wool. Contains no growth factors or cytokines.


Must be supplemented with growth factors and cytokines according to individual user protocols.

Legal Information

Stemline is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

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Z701475 Stem Cells Handbook, S. Sell, ed., Humana Press, 2003, 528 pp., hard cover

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