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S0266 Sigma

Spermidine

BioReagent, for molecular biology, suitable for cell culture, ≥98%

Synonym: 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane

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Properties

Related Categories Biochemicals, Molecular Biology, Molecular Biology Reagents More...
grade   for molecular biology
InChI Key   ATHGHQPFGPMSJY-UHFFFAOYSA-N
product line   BioReagent
assay   ≥98%
form   solid or liquid
refractive index   n20/D 1.479(lit.)
density   0.925 g/mL at 25 °C(lit.)
suitability   suitable for cell culture
foreign activity   DNase, RNase, and protease, none detected
storage temp.   room temp
Gene Information   rat ... Ppm1a(24666)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Caution

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

General description

Spermine is a polyamine involved in cellular metabolism and DNA stabilization. In molecular biology it can be used in buffers for purification of DNA.

Application

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2735PSN2 8 / PGII
WGK Germany 
3
RTECS 
EJ7000000
Flash Point(F) 
233.6 °F
Flash Point(C) 
112 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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S1141 Spermine tetrahydrochloride, BioReagent, for molecular biology

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