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S0664 Sigma

Monoclonal Anti-Syntaxin antibody produced in mouse

clone HPC-1, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, Antibodies to Synaptic Proteins,
species reactivity   rabbit, bovine, rat
application(s)   immunocytochemistry: suitable using monolayer cultures of neonatal retina cells
  microarray: suitable
  western blot: 1:2,000 using crude preparation of synaptic vesicles from rat cerebral cortex
clone   HPC-1, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 35 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Stx1a(116470)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synaptosomal plasma-membrane fraction from adult rat hippocampus.

General description

Syntaxin is a 35kD synaptic protein capable of interacting with the synaptic vesicle protein synaptotagmin. It facilitates the docking and fusion of synaptic vesicles with the plasma membrane. Monoclonal anti-syntaxin antibody can be used to screen λ gt 11 library of rat hippocampus to isolate syntaxin cDNA clones. It can also be used in microarray and immunocytochemistry. Mouse anti-syntaxin antibody reacts specifically with membrane protein syntaxin (35 kD). The product has also shown cross reactivity for rabbit, rat and bovine but not for guinea pig.

Application

Monoclonal anti-syntaxin antibody can be used in immunoprecipitation and immunohistochemistry. It can also be used in western blotting. Further, this product can also be used to label monolayer cultures of neonatal retinal cells and frozen paraformaldehyde fixed tissue sections.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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