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S0937 Sigma

Sucrose Phosphorylase

recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

Synonym: Sucrose:orthophosphate α-D-glucosytransferase

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Properties

Related Categories 2.4.x.x Glycosyltransferases, 2.x.x.x Transferases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
recombinant   expressed in E. coli
form   lyophilized powder
mol wt   mol wt 56 kDa by SDS-PAGE
shipped in   wet ice
storage temp.   −20°C

Description

Biochem/physiol Actions

Enzymatically converts sucrose to D-fructose and D-glucose 1-phosphate.

General description

Sucrose phosphorylase catalyzes the reversible conversion of sucrose (α-D-glucopyranosyl-1,2-β-D-fructofuranoside) and phosphate into D-fructose and α-D-glucose 1-phosphate.

Packaging

250, 1000 units in glass bottle

Unit Definition

One unit will produce 1.0 μmole of D-fructose from sucrose per min with the corresponding reduction of NADP to NADPH at pH 7.6, at 25 °C.

Physical form

Contains sucrose as stabilizer.

Application

Sucrose phosphorylase has been used in a study to assess the enzymatic synthesis of stable, odorless, and powdered furanone glucosides. Sucrose phosphorylase has also been used in a study to investigate the novel transglucosylating reaction with carboxylic compounds.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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