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S0948 Sigma

Anti-SIRT4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-SIR2L4, Anti-Sirtuin (silent mating type information regulation 2 homolog) 4, Anti-sir2-like 4, Anti-sirtuin 4, Anti-sirtuin type 4

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Gene Regulation and Expression,
species reactivity   mouse (predicted), human, rat (predicted)
application(s)   indirect immunofluorescence: 2-4 μg/mL using human SH-SY5Y cells
  western blot: 2-4 μg/mL using whole extracts of HEK-293T cells expressing recombinant human SIRT4
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~34:30 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SIRT4(23409)
mouse ... Sirt4(75387)
rat ... Sirt4(304539)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 302-314 of human SIRT4, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat Sirt4.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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