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  • S1311 - Anti-SIRPα1 (SHPS-1) antibody produced in rabbit

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S1311 Sigma

Anti-SIRPα1 (SHPS-1) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Non-Receptor Tyrosine Kinase Adaptor Protein Antibodies,
species reactivity   rat, mouse
application(s)   immunoprecipitation: suitable
  microarray: suitable
  western blot: 4 μg/mL using RIPA lysates of mouse M1 myeloblast
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous glycerol solution
mol wt   antigen mol wt 100 kDa
  antigen mol wt 130 kDa
  antigen mol wt 50 kDa
  antigen mol wt 65 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... Sirpa(19261)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

fusion protein corresponding to the cytoplasmic domain of rat SHPS-1 (residues 418-481).

General description

Signal regulatory proteins (SIRPs) are a class of transmembrane glycoproteins with extracellular domains resembling the immunoglobulins. The SIRP family consists of three members, SIRPα, SIRPβ and SIRPγ. In rat there are two SIRPβ members, SIRPβ1 and SIRPβ2. SIRP members are ubiquitously present on the surface of all immune system cells. SIRPα (SHP-1) is expressed on neurons, myeloid dendritic cells, mast cells, macrophages and granulocytes. The most studied role of SIRPα is the inhibition of host cell phagocytosis by the macrophages. SIRPα is reportedly involved in clearance of aged erythrocytes, regulation of production of TNF-α induced by LPS, controlling the migration of myeloid cells and maturation of dendritic cells
Anti-SIRPα1 (SHPS-1) recognizes the differentially glycosylated forms of SIRPα1(SHPS-1) of 130, 100 and 65 kDa. It also detects a single band at 50 kDa.

Physical form

Solution in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.

Specificity

The antibody recognizes SIRPα1 (signal-regulatory protein) also known as BIT, SHPS-1, or p84. By immunoblotting, the antibody recognizes the differentially glycosylated forms of SIRPα1 (130, 100 and 65 kDa). It also detects a single band at 50 kDa.

Application

Anti-SIRPα1 may be used in immunoblotting application at a working concentration of 4 μg/mL using mouse myeloblast whole cell lysate. The antibody is suitable for protein microarray and immunoprecipitation.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

WGK Germany  1
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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S1572 Monoclonal Anti-SIRPα/CD172a antibody produced in mouse, ~2 mg/mL, clone SE5A5, purified immunoglobulin, buffered aqueous solution

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