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  • S1312 - Spectrophotometry and Spectrofluorimetry: A Practical Approach, 2nd ed.

S1312 Sigma

Spectrophotometry and Spectrofluorimetry: A Practical Approach, 2nd ed.

  •  ISBN-10 0-19-963812-8

  •  ISBN-13 978-0-19-963812-3



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publication info   M. Gore, ed., Oxford University Press, 2000, 384 pp., soft cover
mfr. no.   Oxford University Press


General description

Optical spectroscopy underpins the day to day operations of most laboratories in the chemical, biological and medical sciences. This edition contains substantially updated and new chapters addressing the principles of the more common applications. In all chapters the emphasis is placed upon the practical aspects, with protocols for test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy, such as: basic enzyme kinetics, ligand binding, data handling, and the more recently established interest in the study of protein and DNA stability

Table of Contents

List of protocols
1. Introduction to light absorption; visible and ultraviolet spectra,
2. Fluorescence principles and measurement,
3. Time resolved fluorescence and phosphorescence spectroscopy,
4. An introduction to circular dichroism,
5. Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods,
6. Steady state kinetics,
7. Spectrophotometric assays,
8. Stopped flow spectroscopy,
9. Stopped flow fluorescence spectroscopy,
10. Stopped flow circular dichroism,
11. Spectrophotometry and fluorimetry of cellular compartments and intracellular processes,
12. Use of optical spectroscopic methods to study the thermodynamic stability of proteins,
13. The use of spectroscopic techniques in the study of DNA stability,
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