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S1402 Sigma

Sulforhodamine B sodium salt

powder, BioReagent, suitable for cell culture

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Properties

Related Categories Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Biochemicals and Reagents, Cell Culture, Luminescent Compounds/Detection, Reagents and Supplements,
product line   BioReagent
form   powder
composition   Dye content, ~75%
solubility   methanol: soluble1 mg/mL
fluorescence   λex 565 nm; λem 586 nm in H2O
suitability   suitable for cell culture

Description

Application

Use as a fluorescent dye to quantify cellular proteins in cultured cells.

Packaging

1, 5, 25 g in glass bottle

Price and Availability

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Sulforhodamine B

Dye content 75 %

Sulforhodamine B acid chloride

suitable for fluorescence, technical

Sulforhodamine B sodium salt

Technical grade

Sulforhodamine B, acid form

laser grade, Dye content 95 %

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
WGK Germany 
2
RTECS 
BP6750000

Protocols & Articles

Articles

Cell Viability and Proliferation

Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an ass...
Mark Frei
BioFiles v6 n5, 17–21
Keywords: Applications, Biofiles, Cell proliferation, Flow cytometry, Mutagens, Reductions, Solvents, Type

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Facilitation of transcutaneous drug delivery and vaccine immunization by a safe laser technology. Chen X, Shah D, Kositratna G, et al. J. Control. Release 159(1), 43-51, (2012)

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The dynamics of giant unilamellar vesicle oxidation probed by morphological transitions. Sankhagowit S, Wu SH, Biswas R, et al. Biochim. Biophys. Acta 1838(10), 2615-24, (2014)

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Cytotoxic activities of some novel benzhydrylpiperazine derivatives. Gurdal EE, Yarim M, Durmaz I, et al. Drug Res. (Stuttg.) 63(3), 121-8, (2013)

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Monitoring temperature with fluorescence during real-time PCR and melting analysis. Sanford LN and Wittwer CT Anal. Biochem. 434(1), 26-33, (2013)

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Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines. Nedel F, Begnini K, Carvalho PH, et al. J. Med. Food 15(11), 955-8, (2012)

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Halomethane-induced cytotoxicity and cell proliferation in human lung MRC-5 fibroblasts and NL20-TA epithelial cells. Nájera-Martínez M, García-Latorre EA, Reyes-Maldonado E, et al. Inhal. Toxicol. 24(11), 762-73, (2012)

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Sulforhodamine B restaining as a whole-cell label allows visualizing one more fluorochrome and its application in assaying protein nucleocytoplasmic distribution. Han F, Fan L, Wang X, et al. Cytometry. A 81(6), 532-40, (2012)

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Characterization of a genotoxic impact compound in Alternaria alternata infested rice as Altertoxin II. Schwarz C, Tiessen C, Kreutzer M, et al. Arch. Toxicol. 86(12), 1911-25, (2012)

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Permeation of insulin, calcitonin and exenatide across Caco-2 monolayers: measurement using a rapid, 3-day system. Gupta V, Doshi N, and Mitragotri S PLoS ONE 8(2), e57136, (2013)

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Additive toxicity of β-amyloid by a novel bioactive peptide in vitro: possible implications for Alzheimer's disease. Garcia-Ratés S, Lewis M, Worrall R, et al. PLoS ONE 8(2), e54864, (2013)

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Conventional caries removal and sealed caries in permanent teeth: a microbiological evaluation. Maltz M, Henz SL, de Oliveira EF, et al. J. Dent. 40(9), 776-82, (2012)

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Transdermal delivery of molecules is limited by full epidermis, not just stratum corneum. Andrews SN, Jeong E, and Prausnitz MR Pharm. Res. 30(4), 1099-109, (2013)

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Polyethylenimine-capped Ag nanoparticle film as a platform for detecting charged dye molecules by surface-enhanced Raman scattering and metal-enhanced fluorescence. Kim K, Lee JW, and Shin KS ACS Appl. Mater. Interfaces 4(10), 5498-504, (2012)

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Reversal of multidrug resistance by morning glory resin glycosides in human breast cancer cells. Gabriela Figueroa-González et al J. Nat. Prod. 75, 93-7, (2012)

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Recovery of skin barrier after stratum corneum removal by microdermabrasion. Andrews S, Lee JW, and Prausnitz M AAPS PharmSciTech 12(4), 1393-400, (2011)

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Comparative analysis of dynamic cell viability, migration and invasion assessments by novel real-time technology and classic endpoint assays. Limame R, Wouters A, Pauwels B, et al. PLoS ONE 7(10), e46536, (2012)

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Inhibition of EGFR-AKT axis results in the suppression of ovarian tumors in vitro and in preclinical mouse model. Loganathan S, Kandala PK, Gupta P, et al. PLoS ONE 7(8), e43577, (2012)

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Bioassay-guided isolation of an alkaloid with antiangiogenic and antitumor activities from the extract of Fissistigma cavaleriei root. Yang Z, Lu W, Ma X, et al. Phytomedicine 19(3-4), 301-5, (2012)

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Symplasmic, long-distance transport in xylem and cambial regions in branches of Acer pseudoplatanus (Aceraceae) and Populus tremula x P. tremuloides (Salicaceae). Sokołowska K and Zagórska-Marek B Am. J. Bot. 99(11), 1745-55, (2012)

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Decolorization of industrial synthetic dyes using engineered Pseudomonas putida cells with surface-immobilized bacterial laccase. Wang W, Zhang Z, Ni H, et al. Microb. Cell Fact. 11, 75, (2012)

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Dental caries area of rat molar expanded by cigarette smoke exposure. Fujinami Y, Nakano K, Ueda O, et al. Caries Res. 45(6), 561-7, (2011)

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Synthesis and biological evaluation of antitumor-active arglabin derivatives. Csuk R, Heinold A, Siewert B, et al. Arch. Pharm. (Weinheim) 345(3), 215-22, (2012)

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Binding of rhodamine B and kiton red S to cucurbit[7]uril: density functional investigations. Khedkar JK, Jagtap KK, Pinjari RV, et al. J. Mol. Model. 18(8), 3743-50, (2012)

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Desipramine protects neuronal cell death and induces heme oxygenase-1 expression in Mes23.5 dopaminergic neurons. Lin HY, Yeh WL, Huang BR, et al. PLoS ONE 7(11), e50138, (2012)

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Nanoformulation of siRNA silencing Bcl-2 gene and its implication in cancer therapy. Jagani HV, Josyula VR, Hariharapura RC, et al. Arzneimittelforschung 61(10), 577-86, (2011)

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Dispersion serial dilution methods using the gradient diluter device. Walling L, Schulz C, and Johnson M Assay Drug Dev. Technol. 10(6), 507-13, (2012)

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Human Noxin is an anti-apoptotic protein in response to DNA damage of A549 non-small cell lung carcinoma. Won KJ, Im JY, Yun CO, et al. Int. J. Cancer 134(11), 2595-604, (2014)

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Beil. 19,V,11,311

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TOX6 In Vitro Toxicology Assay Kit, Sulforhodamine B based

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