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S1686 Sigma

Monoclonal Anti-v-Src antibody produced in mouse

clone 327, purified immunoglobulin, lyophilized powder

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphorylation Enzymes,
species reactivity   mouse, human, rat
application(s)   immunoprecipitation: suitable
  western blot: 2-5 μg/mL using COLO201 cell extract
clone   327, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG1κ
mol wt   antigen mol wt ~56 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... SRC(6714)
mouse ... Src(20779)
rat ... Src(83805)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

Src protein (p60src).

General description

Src family kinases are a group of structurally related non-receptor protein tyrosine kinases that can lead to a variety of cellular responses including differentiation and proliferation. All the members have an amino terminal that has the kinase function and the C-terminal that regulates the kinase function. The amino terminal has Src homology 4 domain (SH4), containing a myristylation signal sequence essential for membrane localization; conserved SH3 and SH2 domains, which mediate protein-protein interactions, separated from the SH4 by a unique region; a catalytic kinase domain. The major difference between v-Src and c-Src is the structure of the C terminal. Src is involved in the regulation of many cellular functions such as migration, adhesion, gene transcription, cell cycle, apoptosis and differentiation. Few signalling pathways through which Src regulates these processes are PI3K, STAT3, FAK and paxillin. Src overexpression is observed in human cancers.
Monoclonal Anti-v-Src reacts with active Src kinase from human, mouse, and rat detecting a band of approximately 56 kDa.

Physical form

Lyophilized with 100 μg bovine serum albumin.

Application

The recommended anti-v-Src antibody concentration is 2-5 μg/mL for immunoblotting using a COLO201 cell extract and chemiluminescence detection. The antibody is suitable for immunoprecipitation.

Analysis Note

p60src may appear as a doublet due to phosphorylation.

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