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S1797 Sigma

SOC Medium

For use in transformation

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Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Components

20 g/L Tryptone
5 g/L Yeast Extract
4.8 g/L MgSO4
3.603 g/L dextrose
0.5g/L NaCl
0.186 g/L KCl

Application

SOC Medium is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations. Use of SOC maximizes the transformation efficiency of competent cells.

Features and Benefits

SOC liquid provides:
• Filter-sterilized, ready-to-use format
• Convenient package sizes
• Standard formulation

General description

SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Price and Availability


Microbial Media

Oxford Genetics

New Competent Cells

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Safety & Documentation

Safety Information

WGK Germany 
3

Protocols & Articles

Articles

Competent Cells

Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment. Once it enters the cytoplasm, the genetic material may be degraded by nucleases i...
Keywords: Antibiotics, Cloning, Detergents, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Molecular biology, Peptide synthesis, transformation

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Protocols

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transformation Protocols

From our library of Protocols, Sigma-Aldrich presents Transformation Protocols
Keywords: Antibiotics, Detergents, Electrophoresis, Gel electrophoresis, Mass spectrometry, Peptide synthesis, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

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Keywords: Antibiotics, Cell culture, Culture media, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Plant biotechnology, Vitamins, transformation

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