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S1797 Sigma

SOC Medium

For use in transformation

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

20 g/L Tryptone
5 g/L Yeast Extract
4.8 g/L MgSO4
3.603 g/L dextrose
0.5g/L NaCl
0.186 g/L KCl

Application

SOC Medium is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations. Use of SOC maximizes the transformation efficiency of competent cells.

Features and Benefits

SOC liquid provides:
• Filter-sterilized, ready-to-use format
• Convenient package sizes
• Standard formulation

General description

SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Which bacterial culture medium is the best choice for my application?
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol.  Generally: LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics. LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content. Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium. SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells. SOC is used for initial growth of competent cells and the transformation procedure.
Why is SOC Medium (S1797) used to recover bacterial cells before transformation?
SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation.  The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.
Show more questions
Protocols & Articles

Articles

Competent Cell Protocols

Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment. Once it enters the cytoplasm, the genetic material may be degraded by nucleases i...
Keywords: Antibiotics, Cloning, Detergents, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Molecular biology, Peptide synthesis, transformation

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Protocols

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transformation Protocols

From our library of Protocols, Sigma-Aldrich presents Transformation Protocols
Keywords: Antibiotics, Detergents, Electrophoresis, Gel electrophoresis, Mass spectrometry, Peptide synthesis, transformation

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

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Keywords: Antibiotics, Cell culture, Culture media, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Vitamins, transformation

Peer-Reviewed Papers
15

References

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