• USA Home
  • S1813 - S-Gal®/LB Agar/Kanamycin Blend

S1813 Sigma

S-Gal®/LB Agar/Kanamycin Blend





Ingredients (g/L)
Tryptone, 10
Yeast extract, 5
Sodium chloride, 5
Agar, 12
S-Gal, 0.25
Ferric ammonium citrate, 0.5
IPTG, 0.05
Kanamycin, 0.035


S-Gal®/LB Agar/Kanamycin Blend is designed for identification of recombinant bacterial clones with the lac+ phenotype in those systems that utilize kanamycin as a selection agent.

Features and Benefits

S-Gal/LB Agar/Kanamycin Blend is a complete, autoclavable and/or microwaveable media blend that contains everything necessary to do color selection.

Other Notes

Autoclavable, microwavable, complete with S-Gal®, IPTG and kanamycin.

Related Products: IPTG (Isopropyl β-D-thiogalactopyranoside, I6758), Ampicillin (A2804), Kanamycin (K0879), Chloramphenicol (C7795), Tetracycline (T8032)


Suspend contents of one packet in 500 mL distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124 °C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Kanamycin is already included, but if additional antibiotics are desired, they should be added following autoclaving or microwaving, after cooling to 48-52 °C.

Legal Information

S-GAL is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Related Products

related product

Product #


Add to Cart

C4478 S-Gal®/LB Agar Blend, reagent for selection of recombinant bacterial clones
B3928 Blue-White Select Screening Reagent, for selection of recombinant bacterial clones
B8931 Magenta-Gal, reagent for selection of recombinant bacterial clones
S9811 S-Gal®, reagent for selection of recombinant bacterial clones
S7313 S-Gal® sodium salt, reagent for selection of recombinant bacterial clones
16658 X-GalNAc, reagent for selection of recombinant bacterial clones
B6650 X-GlcA, reagent for selection of recombinant bacterial clones
B2904 Bluo-Gal, reagent for selection of recombinant bacterial clones

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?