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S1813 Sigma

S-Gal®/LB Agar/Kanamycin Blend

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Description

Components

Ingredients (g/L)
Tryptone, 10
Yeast extract, 5
Sodium chloride, 5
Agar, 12
S-Gal, 0.25
Ferric ammonium citrate, 0.5
IPTG, 0.05
Kanamycin, 0.035

Application

S-Gal®/LB Agar/Kanamycin Blend is designed for identification of recombinant bacterial clones with the lac+ phenotype in those systems that utilize kanamycin as a selection agent.

Features and Benefits

S-Gal/LB Agar/Kanamycin Blend is a complete, autoclavable and/or microwaveable media blend that contains everything necessary to do color selection.

Other Notes

Autoclavable, microwavable, complete with S-Gal®, IPTG and kanamycin.

Related Products: IPTG (Isopropyl β-D-thiogalactopyranoside, I6758), Ampicillin (A2804), Kanamycin (K0879), Chloramphenicol (C7795), Tetracycline (T8032)

Reconstitution

Suspend contents of one packet in 500 mL distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124 °C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Kanamycin is already included, but if additional antibiotics are desired, they should be added following autoclaving or microwaving, after cooling to 48-52 °C.

Legal Information

S-GAL is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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