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  • S1940 - Anti-phospho-Src (pTyr418) antibody produced in rabbit

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S1940 Sigma

Anti-phospho-Src (pTyr418) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-pp60src(pY418)

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphotyrosine (pTyr),
species reactivity   chicken, human, mouse, rat
application(s)   western blot: 1:1,000 using human platelets or mouse NIH3T3 cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... SRC(6714)
mouse ... Src(20779)
rat ... Src(83805)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

The sequence is conserved in human, mouse, chicken and frog.

synthetic phosphopeptide derived from the region of src that contains tyrosine 418, based on the sequence of human src.

General description

Src is a proto-oncogene that regulates neuronal functions, such as nerve cell differentiation. The Src kinase family structurally comprises of SH2, SH3 and kinase domains, as well as a negative regulatory C-terminal tail. Src has also been implicated in functions such as cell growth, development and cell-cell interactions . Anti-phospho-Src p(Tyr418) antibody is specific for human, mouse, rat and chicken Src (60 kDa).

Physical form

Solution in Dulbecco′s phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/ml BSA (IgG and protease free) and 0.05% sodium azide.

Application

Anti-phospho-Src p(Tyr418) antibody is suitable for use in western blot (1:1,000 using human platelets or mouse NIH3T3 cells). The product may also be used for immunoprecipitation (1:1000 using HEK293 or HEL cell lysates ).

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