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S2415 Sigma

Streptavidin−Iron Oxide Particles from Streptomyces avidinii

buffered aqueous suspension, ~1.5 μm

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Properties

Related Categories Affinity Chromatography, Antibodies, Avidin Biotin Matrices, Biotin/Avidin, Molecular Biology,
form   buffered aqueous suspension
concentration   0.8-1.2 mg/mL
matrix   Superparamagnetic Iron Oxide Particles
matrix activation   amine
particle size   ~1.5 μm
capacity   ≥2 μg/mg binding capacity (poly(A) RNA)(using biotinylated oligo(dT)20)
foreign activity   DNase and RNase, none detected
storage temp.   2-8°C

Description

Application

Used for the purification of biotin containing proteins or DNA binding proteins

Streptavidin iron oxide particles are used in protein chromatography, affinity chromatography, and recombinant protein expression and analysis. Streptavidin iron oxide particles have been used to study imaging of cellular spread on a three-dimensional scaffold by means of a novel cell-labeling technique for high-resolution computed tomography. Streptavidin iron oxide particles have also been used to study in vitro biomedical applications of functionalized iron oxide nanoparticles.

Physical form

Suspension in 0.85% NaCl, 0.01 M phosphate, pH 8.0, containing 0.1% bovine serum albumin, 0.1% sodium azide

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Search 80,000 Antibodies at Sigma-Aldrich

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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