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S2519 Sigma

Anti-Soy Protein antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Miscellaneous, Plant Biology, Primary Antibodies,
biological source   rabbit
antibody form   fractionated antiserum
clone   polyclonal
form   buffered aqueous solution
species reactivity   soy
application(s)   dot blot: 1:500
  indirect ELISA: 1:5,000
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Antigen Background

Soy protein in a variety of forms is the basis of many low cholesterol foods and may also be incorporated in animal meat products.

Immunogen

renatured soy protein extracted from soy acetone powder with hot urea.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Soy Protein antibody produced in rabbit was used for soy quantification in natural rubber latex glove extracts by ELISA at 1:2000 dilution. It was used as a control in supershift analysis of growth-hormone-induced protein binding to lactogenic hormone-responsive region.

Biochem/physiol Actions

Soy protein is a rich source of isoflavones that act as estrogen analogs. They exert both estrogenic and anti-estrogenic effects depending on the tissue in which they act. Isoflavones effect the lipid and bone metabolism and may act as a potential alternative to estrogen in post-menopausal women.

General description

Some cross-reaction is observed with extracts from wheat flour and ovalbumin; no cross-reaction is observed with extracts from maize, rice, potato flour, casein, or meat.

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RIDADR 
NONH for all modes of transport
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Peer-Reviewed Papers
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