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  • S2532 - Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse

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S2532 Sigma

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse

clone SH-B1, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   sheep, rat, pig, goat, rabbit, feline, human, canine, bovine
application(s)   dot blot: suitable using denatured-reduced preparations
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using protease-digested sections of human tongue
  indirect ELISA: suitable
  microarray: suitable
clone   SH-B1, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... S100B(6285)
rat ... S100b(25742)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

bovine brain S-100b

Specificity

This antibody recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B1 is independent of Ca2+. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues.

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