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S2647 Sigma

Spectinomycin dihydrochloride pentahydrate

powder, γ-irradiated

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Properties

Related Categories Aminoglycosides, Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antibiotics by Application,
grade   for molecular biology
InChI Key   DCHJOVNPPSBWHK-UXXUFHFZSA-N
sterility   γ-irradiated
form   powder
solubility   H2O: soluble at 
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   2-8°C
suitability   nonselective for Escherichia coli and Coliforms

Description

Biochem/physiol Actions

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Caution

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Aminoglycosides are a well-known class of antibiotics that are routinely monitored in animal-derived foods. Many countries have instituted regulatory limits for aminoglycosides. There is growing conc...
Emily Barrey, Olga Shimelis, K. G. Espenschied
Reporter US, Volume 32.2
Keywords: Antibiotics, High performance liquid chromatography, Mass spectrometry, Separation, Size-exclusion chromatography, Solid phase extractions

Peer-Reviewed Papers
15

References

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S0692 Spectinomycin, Ready Made Solution, 100 mg/mL in DMSO/H2O, 1:1, 0.2 μm filtered
S4014 Spectinomycin dihydrochloride pentahydrate, powder, BioReagent, suitable for cell culture

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