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S3014 Sigma

Sodium chloride

for molecular biology, DNase, RNase, and protease, none detected, ≥98% (titration)

Synonym: Halite

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Description

Other Notes

Easily compare specifications for Sodium Chloride products with the Sodium Chloride specification table.

Packaging

1, 6×1 kg in poly bottle

5, 10 kg in poly drum

500 g in poly bottle

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
VZ4725000
Protocols & Articles

Articles

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Restriction Enzyme Cloning Manual Buffer Recipes

Tip 1: DNA is quite stable in TE buffer at 4ºC. If stored in elution buffer or water then freezing at -20 ºC is advised.
Keywords: Phase transitions, Sequencing, Sterilizations

Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Peer-Reviewed Papers
15

References

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