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S3062 Sigma

Anti-α-Synuclein antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-SNCA

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Parkinson’s Disease Research, Cell Biology, Cell Signaling and Neuroscience,
species reactivity   rat, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid treated sections of Alzheimer′s Disease brain
  microarray: suitable
  western blot: 1:1,000 using extract of rat brain homogenate
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 19 kDa
storage temp.   −20°C
Gene Information   human ... SNCA(6622)
rat ... Snca(29219)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to a sequence near the C-terminus of human α-synuclein (amino acids 111-132 with C-terminally added lysine) conjugated to KLH. This sequence is highly conserved (90% homology) in mouse and rat α-synuclein. This sequence has no homology with β- and γ-synuclein.

General description

The synucleins are a family of soluble presynaptic proteins that are abundant in neurons and include α-synuclein, β-synuclein, and γ-synuclein. Human α-synuclein (also known as the non-amyloid component of plaques precursor protein or NACP) is a 140-amino acid polypeptide that is encoded by a gene on chromosome 4. It was originally isolated from plaques of Alzheimer’s disease (AD) brains as a 19 kDa protein precursor of the highly hydrophobic 35-amino acid peptide, nonamyloid component (NAC) of plaques. The NAC can self-aggregate into fibrils and induce aggregation of the β-amyloid peptide. α-Synuclein is highly abundant in presynaptic terminals, and is a major component of Lewy bodies (LBs). Pathogenic point mutations in the α-synuclein gene are linked to familial Parkinson’s disease (PD) in rare kindreds. However, most neurodegenerative disorders with LBs are associated with abnormal accumulation of wild-type, α-synuclein. α-Synuclein is expressed primarily in brain, but is also expressed in low levels in all tissues examined except liver.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Rabbit polyclonal anti-α-Synuclein antibody recognizes human and rat α-synuclein. Staining of α-synuclein in immunoblotting is specifically inhibited by the immunizing peptide

Application

Rabbit polyclonal anti-α-Synuclein antibody is used to tag α-Synuclein for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques, such as immunoblotting. It is used as a probe to determine the presence and roles of α-Synuclein in plaques of Alzheimer’s disease (AD) brains.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Parkinson's Disease

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease, affecting more than 6 million people worldwide. PD is a slowly progressing motor system neurod...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 16–20
Keywords: Alzheimer Disease, Apoptosis, Chromatin immunoprecipitation, Clinical, Degradations, Dopamine agents, Gene expression, Parkinson Disease, Polymorphisms

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Peer-Reviewed Papers
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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