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S3264 Sigma

Sodium phosphate dibasic

for molecular biology, ≥98.5% (titration)

Synonym: sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffers / Buffer Salts,
Quality Level   PREMIUM
grade   for molecular biology
InChI Key   BNIILDVGGAEEIG-UHFFFAOYSA-L
assay   ≥98.5% (titration)
form   powder
pH   8.9-9.2 (25 °C, 50 g/L in water)
pKa (25 °C)   (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)
solubility   H2O: soluble100 mg/mL
anion traces   chloride (Cl-): ≤40 ppm
cation traces   Fe: ≤20 ppm
  heavy metals (as Pb): ≤10 ppm
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase, RNase and protease, none detected

Description

Packaging

1 kg in poly bottle

250, 500 g in poly bottle

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
UN 3077 9 / PGIII
WGK Germany 
1
RTECS 
WC4500000
Protocols & Articles

Articles

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

TRI Reagent® Protocol

1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per 50–100 mg of tissue) in a Polytron® or other appropriate homogenizer.
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

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