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S3887 Sigma

Streptavidin–β-Galactosidase conjugate

streptavidin 1-2 units/mg protein, lyophilized powder

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Properties

Related Categories Antibodies, Biotin/Avidin, Streptavidin Reagents, Supplementary Products
form   lyophilized powder
composition   Protein, ≥80% biuret
conjugate   beta-galactosidase tagged
storage temp.   −20°C

Description

Packaging

0.5, 1 mg in glass bottle

Preparation Note

Streptavidin and β-galactosidase grade VIII are conjugated by a modification of the method of O′Sullivan and purified by gel filtration.

Specificity

Streptavidin–β-Galactosidase conjugate is reactive with biotin labeled molecules.

Physical form

Contains Tris buffer salts.

Application

Streptavidin–β-Galactosidase conjugate may be used in conjunction with biotin labeled molecules in techniques such as ELISA, immunohistochemistry, protein blotting or with biotinylated DNA probes. It provides a convenient yet highly sensitive and specific detection system.

General description

Streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii, is a very high affinity and specificity non-covalent biotin binding protein that is used in a wide range of biochemical/immunological assays such as western blot and ELISAs. It is a component of the streptavidin-biotin system; wherein biotin is conjugated to a target molecule that is later detected by a signal tagged streptavidin. Streptavidin may be covalently tagged with a range of enzymes that develop chromogenic sustrates or fluorescent molecules to support detection systems.
β-galactosidase may be used in genetic, molecular and immunologic detection systems. Active β-galatosidase cleaves the detection molecule X-gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) to form an intense blue product that may be detected by chromogenic techniques.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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