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S400HR Sigma

Sephacryl®

400-HR, MW range 20-8000 kDa (globular proteins)

Synonym: Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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Properties

Related Categories Analytical/Chromatography, Gel Filtration Chromatography, Gel Filtration Media, Molecular Biology, Protein Chromatography,
form   suspension (20% aqueous ethanol)
feature   autoclavable
  stability stable to 0.2M NaOH
matrix   crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
particle size   25-75 μm (wet)
pore size   10-2000 kDa MW range (dextrans)
  20-8000 kDa MW range (globular proteins)
compatibility   mode of use size exclusion chromatography
storage temp.   2-8°C

Description

Application

For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans.

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.

General description

• Supplied swollen, suspended in 20% aqueous ethanol
• Autoclavable
• Stable to 0.2N sodium hydroxide (NaOH)
• Store at refrigerated temperature (2-8 °C)

Packaging

100, 250, 750 mL in glass bottle

Legal Information

Sephacryl is a registered trademark of GE Healthcare

Price and Availability


Tools for Large Molecule BioAnalysis

Detect Your Unseen Protein
Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning
Hazard statements 
RIDADR 
NA 1993 / PGIII
WGK Germany 
1
Flash Point(F) 
100.4 - 109.4 °F
Flash Point(C) 
38 - 43 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Pharmaceutical, Purification, Separation

Peer-Reviewed Papers
15

References

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