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S4045 Sigma

Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse

clone SC-35, ascites fluid

Synonym: Anti-SC-35, Anti-SC35

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for RNA Regulation and Translation,
species reactivity   rat, newt, frog, human, Drosophila
application(s)   electron microscopy: suitable
  immunocytochemistry: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  indirect immunofluorescence: 1:2,000 using cultured human fibroblasts
clone   SC-35, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 35 kDa (a doublet)
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SFRS2(6427)
rat ... Sfrs2(494445)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

partially purified mammalian splicesome.

General description

Nuclear pre-mRNA splicing takes place in a multi-component structure termed a spliceosome. Major subunits of spliceosomes are U1, U2, U4/U6 and U5 small nuclear ribonucleoproteins (snRNP′s). In addition to the snRNP′s, a number of protein factors have been identified which are required for spliceosome assembly and splicing. For example, the protein factors U2AF, SF2 and SF3 are required for the binding of the U2 snRNP to the intron branch-point and for assembly of the pre-splicing complex. Two other non-snRNP splicing factors, SF2/ASF and SC-35 (splicing component of 35 kD, also termed PR264), are both required for the first step of splicing and spliceosome assembly. SF2/ASF and SC-35 are also involved in 5N splice site selection of alternatively spliced pre-mRNA′s.
The essential non-snRNP splicing factor SC-35 displays a speckled distribution in the nucleus that co-localizes with snRNPs, but unlike snRNPs, SC-35 does not give diffuse nuclear labelling. In the nucleus, snRNPs are concentrated in coiled bodies and in the speckled regions, whereas SC-35 is found in speckles but not in coiled bodies.

Specificity

Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli. Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification.

Application

Monoclonal Anti-Splicing Factor SC-35 may be used for the localization of SC-35 using ELISA, immunohistology and immunoelectronmicroscopy, for inhibition and depletion of splicing activity in nuclear extracts, and for immunoaffinity purification and immunoprecipitation.
Indirect immunofluorescence: a dilution of at least 1:2,000 was determined by staining cultured human fibroblasts.

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